转染肿瘤坏死因子及反向多药耐药基因对乳腺癌细胞耐药基因表达的影响.pdfVIP

·2128· ·论著· 转染肿瘤坏死因子及反向多药耐药基因对乳腺癌细胞 耐药基因表达的影响 陈亦欣，文飞球，申维玺，叶建增 【摘要l 同时导入乳腺癌耐药细胞株MCF一7／ADR中进行表达，应用荧光显微镜直接观察融合蛋白的表达情况，RT—PcR和免 pDsRed2一’硪F—ct载体的细胞可见到红色及绿色荧光。转染后的细胞在mRNA水平及蛋白水平明显降低了耐药细胞 因治疗联同免疫治疗逆转乳腺癌耐药建立了很好的基础。 【关键词】 多药耐药基因；肿瘤坏死因子Q；乳腺肿瘤 737．9 【中图分类号】R 【文献标识码】A 【文章编号】1007—9572(2008)12—2128一04 Effectsof MDRlC,eneTransfecttononthe ofMDRlGeneinBreastCancero山Line TNT—nandReverse Expression a1．Cancer SecondClinical nan CHEN Institute，the ofJr Yi—xin。WENFd—qiu，sHENWet—xi．et Hospital University。 Shenzhen 518020．China To therecombinantvectorsofreverseenhancedfluorescent Objective green protein(EGFP)with 【Abstract】 express reYerse resistance redfluorescent TNF—OL protein(DsRed2)withgene(pDsRed2一TNF— muhidrug l(pEGFP—MDRI)and effectson ofMDRI d)in resistantbreastcancercelllineMCF一7／ADR，andtoobservetheir the multidrug expression gene． vector well嬲recombinantvectorof MethodsToconstructtherecombinantofpEGFP—MDRlgene．as pDsRed2一TNF— andDNArecombinant recombinantvectorswereintroducedinto resistantbreast agenebyRT—PCR techniques．The muhidrug MCF一7／ADRandthe ofthefusion Was observedfluorescent ex· cancercellline expression proteinsdirectly by microscope．The ofMDRl一mRNAand andafterthetransfectionwereobservedRT—PCRandim· pressions P—glycoprotein(P—gP)before by