双波长HPLC同时测定安坤种子丸中阿魏酸和芍药苷含量.pdfVIP

2013年 9月第 20卷第 9期 中国中医药信息杂志 ·55· 双波长 HPLC 同时测定安坤种子丸中 阿魏酸和芍药苷含量 张小华,胡君茹,马琴国,刘效栓,李喜香 甘肃省中医院药学部,甘肃 兰州 730050 摘要：目的 采用双波长高效液相色谱法同时测定安坤种子丸中阿魏酸和芍药苷2种有效成分的含量。方法 采 用Waters SymmetryShield-C18 柱(4.6 mm×250 mm,5 µm),以乙腈-0.1%磷酸溶液为流动相梯度洗脱,流速1.0 mL/min, 检测波长分别为230、323 nm。结果 阿魏酸、芍药苷线性范围分别为0.058 2～0.582 4 µg(r ＝0.999 4)、1.664～ 16.64 µg(r ＝0.999 6),平均加样回收率分别为 97.77%(RSD＝1.88%)、98.84%(RSD＝1.96%)。结论 所建立的方法 可对安坤种子丸中阿魏酸和芍药苷准确、快速地进行定量检测,可用于其质量控制。 关键词：安坤种子丸；阿魏酸；芍药苷；双波长高效液相色谱法 DOI：10.3969/j.issn.1005-5304.2013.09.019 中图分类号：R284.1 文献标识码：A 文章编号：1005-5304(2013)09-0055-03 Simultaneous Determination of Ferulic Acid and Paeoniflorin in Ankong Zhongzi Wan by Dual Wavelength HPLC ZHANG Xiao-hua, HU Jun-ru, MA Qin-guo, LIU Xiao-shuan, LI Xi-xiang (Department of Pharmacy, Gansu Province Hospital of TCM, Lanzhou 730050, China) Abstract ：Objective To determine ferulic acid and paeoniflorin in Ankong Zhongzi Wan by HPLC under dual wavelength ultraviolet detection. Methods Ferulic acid and paeoniflorin were separated by Waters SymmetryShield-C18 column (4.6 mm ×250 mm, 5 µm) with gradient elution of acetonitrile-0.1% phosphoric acid as the mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was 230 nm and 323 nm. Results The linear relationship of ferulic acid and paeoniflorin was good in the range of 0.0582-0.582 4 µg (r ＝0.999 4) and 1.664-16.64 µg (r ＝0.999 6), and the average recovery rate was 97.77% (RSD＝1.88%) and 98.84% (RSD＝1.96%), respectively. Conclusion The method is accurate and quick for determining the two effective components in Ankong Zhongzi W