培养法与PCR法对肺炎支原体临床检测及耐药分析.pdfVIP

·论 善· J Med No．2 Res，Feb2013，V01．42 培养法与PCR法对肺炎支原体临床检测及耐药分析 王敏郑业焕付光宇 摘 要 目的 考察培养法与PcR法用于临床检测肺炎支原体的差异及相关药敏分析。方法用培养法和PcR法平行检 测收集自河南省人民医院与郑州人民医院的433份临床呼吸道样本。结果培养法阳性率为22．86％，PCR法阳性率为 酯类耐药情况普遍高于其他抗生素。结论培养法检测肺炎支原体技术日趋成熟，并且与药敏检测可以同步进行，对临床肺炎 支原体感染的检测和耐药性监测具有重要意义，适合各级医院的推广使用。 关键词 肺炎支原体PCR培养耐药 ofCljnjcal andResistance theMethodsofCuIture Analysis and Tes廿ng of弄移c叩缸删口P弹P口成on面孵by PCR．矸-wlgmi乃，．孙e，‘g娩^配n凡， F“GMⅡr。gyu．Depnrt，凡en￡Q厂Re印irⅡ￡07y肘edicine，Z^e，堆弘^ouPeopfe’s．—r0印ifof，月台凡nn‘≠5DD03，C^i凡Ⅱ Abstract To thediffbrencebetweenthemethodsofcultureandPCRinclinical and ObjectiVeinvestigate applicationsanalyze of and culturePCR．MethodsAtotalof433clinical were drugsusceptibilitytestingMycoplasmapneumoniaeby respiratoryspecimens collectedfromthe ofHenanProvinceand andtestedbothmethodsofcultureandPCR．Results Hospital ZhengzhouPeople’sHospital by The rateofculturemethodwas the rateofPCRmethodwas24．94％．Therewasno di妇erence positlve 22．86％，and significant positive betweenthetwodetectionmethods test(P0．05)． ofantibioticre· bychi—square Mycoplasmapneumoniaepresentedvaryingdegrees in resistanee was other ofantibioties．Conclu· sistancecIinical macIDlide than detection，amongwhieh，the against generallyhigher types sionThe ofdetectionof mediumculturemethodis Anditcouldbecarriedout