超速冻存小鼠卵巢组织的可行性与研究.pdf

! 超运盗在d：星塑墨塑堡鳇互踅性亟容 超速冻存小鼠卵巢组织的可行性研究 中文摘要 [摘要] 目的探讨超速冷冻和慢速冷冻技术对小鼠卵巢组织冷冻损伤程度的 影响。方法使用旺染色和TUNEL技术分别定量评估冻存后的始基卵泡及初级 卵泡的形态学及DNA片断的变化。用免疫组化方法检测冻存复苏后的小鼠卵巢组 (PO．001)，两冻存组之问无显著差异(乃O．05)aA、B和c三组中，正常形态 初级卵泡的凋亡比率无显著差异(PO．05)，但与对照组相比存在明显差异 冻对小鼠卵巢组织损伤程度的影响无明显差异。超速冷冻可能是一种简便可行、 较理想的冷冻技术。 [关键词] 冻存：卵泡；小鼠卵巢；凋亡；缝隙连接；Cx43；Cx37 ! 塑蓝盗在d!鼠鲤基塑墨鲍豆堑性班塞 The ofmurineovaries feasibilitystudy cryopreservationusing method ultrarapidfreezing To the ofintactmurineovaries 【Abstract】Objectivestudy feasibility methodMethodsIntactmouseovaries cryopreservation freezing usingultrarapid were methodandslow method．The cryopreservedusingultrarapid freezing were alterationsof folliclesafterfrozen-thawed morphological primordial procedure evaluated of follicleswas byhistologicalexamination，theapoptosisprimordial evaluatedtheTUNEL theultrastructuralWeleobserved by technique，and changes by electron ofCx43andCx37in TEM(transmissionmicroscopy)．Theexpression follicleswereexamined inthe preantral immunohistochemically in mouseovariesResultsTheratiosof folliclesnormal primordial morphology were