两种方法制作大鼠大脑中动脉栓塞再灌注模型比较.docVIP

两种方法制作大鼠大脑中动脉栓塞再灌注模型比较 作者：厉建元 张亚卓 姚维成 桂松柏 孙梅珍 【摘要】 目的 制作短暂性大鼠大脑中动脉栓塞再灌注模型，比较颈总动脉进线法和颈外动脉进线法(改良LONGA法)优劣。方法 颈总动脉进线法和改良LONGA法栓塞大鼠大脑中动脉，栓塞2 h后再灌注24 h，以LONGA法标准对模型评分，并对梗死体积进行测定， 7 d时流式细胞仪检测细胞凋亡情况。结果 改良LONGA法和颈总动脉进线法神经行为学评分分别为2.30±0.82和1.00±0.63，两种方法比较差异有显著性（t=6.886,Plt;0.05）；两法脑梗死体积相比差异有显著性(t=3.96～4.45,Plt;0.05)；两法细胞凋亡率比较差异有显著性（F=348.46，q=22.89,Plt;0.05）。结论 与颈总动脉进线法相比，改良LONGA法大鼠神经行为学评分高、脑梗死体积大、凋亡细胞数多，模型稳定性好。 【关键词】 脑中动脉；再灌注损伤；细胞凋亡；大鼠 ［ABSTRACT］ Objective To make a transient middle cerebral artery occlusion model in rats, and to compare the methods of occlusion of common carotid artery and LONGA’s technique. Methods The cerebral artery occlusion was obtained via blockage of either common carotid artery (CCA) or external carotid artery (modified LONGA’s group). After two hours of artery occlusion and 24 hours of reperfusion, the rats were evaluated by LONGA’s score standard. The volume of cerebral infarction was estimated by TTC staining. The number of apoptotic cells was detected by flow cytometry after seven days of occlusion. Results The neurological behavior scorings in modified LONGA’s group and CCA group were 2.30±0.82 and 1.00±0.63, respectively (t=6.886,Plt;0.05). The infarction volume between the two groups was significantly different (t=3.96-4.45,Plt;0.05). The number of apoptotic cells was significantly different between the two groups (F=348.46,q=22.89,Plt;0.05). Conclusion Compared with the CCA occlusion group, the neurological behavior scoring of modified LONGA’s group was higher, the infarction volume was larger, the number of apoptotic cells was greater, and the stability was better. ［KEY WORDS］ Middle cerebral artery; Reperfusion injury; Apoptosis; TTC staining; Rats 大脑中动脉栓塞再灌注模型是局灶性脑缺血的标准模型。线栓法制作该模型，具有不开颅、效果肯定且可准确控制缺血及再灌注时间，便于分析神经元对缺血敏感性、耐受性及研究再灌注损害和治疗时间窗选择的优点，受广泛关注［1~5］。现将我们制作模型体会报告如下。 1 材料和方法 1.1 实验动物 清洁级3月龄Sprague-Dawley(SD)雄性大鼠102只，体质量260～300 g，购于北京市维通利华公司。栓塞再灌注模型分为：对照组（6只）