中华物理医学与康复志2008年5月第30卷第5期Chin J Phys.doc

中华物理医学与康复杂志2008年5月第30卷第5期Chin J Phys Med Rehabil, May 2008, Vol.30, No.5·基础研究· 不同时程游泳运动对抑郁症模型大鼠 行为及血清皮质醇的影响何松彬王小同唐闻捷 【摘要】目的观察不同时程游泳运动对抑郁症模型大鼠行为及血清皮质醇的影响。 方法选取48只SD大鼠随机分为3个大组，每个大组各设3个亚组（每个亚组6只大鼠），第一大组观察造模时运动对大鼠的影响（造模伴运动组），包括空白对照组、造模对照组、造模伴运动组；第二大组观察造模后运动对大鼠的影响（造模后运动组），包括空白对照组、造模对照组、造模后运动组；第三大组观察造模前运动对大鼠的影响（造模前运动组），包括空白对照组、造模对照组、造模前运动组（造模后运动组与造模前运动组为同一组）。所有造模大鼠均给予21 d慢性轻度不可预见性应激，记录大鼠旷野试验、糖水试验相关数据，用放射免疫法测定血清皮质醇含量。 结果造模可使大鼠出现糖水摄入减少、糖水偏爱度降低，在旷野试验中水平运动能力减弱、垂直运动能力减弱、排粪便数增加。造模伴运动组大鼠上述现象得到抑制，造模前运动组及造模后运动组的大鼠均未能显示抑制现象，造模可使大鼠血皮质醇含量升高，造模伴运动组及造模前运动组能有效降低血清皮质醇含量，其中造模伴运动组尤为明显。 结论不同时程的游泳运动对抑郁症模型大鼠行为学及血清皮质醇具有不同的影响作用；运动可以降低血清皮质醇含量，并且具有一定的累积效应；运动对抑郁症状的改变以即刻作用为主，没有长期累积作用。 【关键词】运动训练；抑郁症；大鼠；行为学；血清皮质醇 Effects of exercise on behavior and serum cortisol in depression due to chronic mild stressHE Songbin, WANG Xiaotong, TANG Wenjie. Wenzhou Medical College, Wenzhou 325000, China 【Abstract】Objective To investigate the effects of exercise on behavior and serum cortisol levels in depression caused by chronic, unpredictable, mild stress (CUMS). MethodsFortyeight male SpragueDawley rats were randomly divided into 3 groups: A, B and C. Each group was then divided into 3 subgroups: a blank control subgroup, a model subgroup, and an intervention subgroup. CUMS such as unpredictable forced swimming in cold water, electric stimulation of the animals feet and shaking of the cage was used to establish a model of depression. Swimming exercise was administered as a therapeutic intervention. In group A it was administered while the animals were under stress, while in groups B and C, exercise was administered before and after the depression model had been established. Openfield test and fluid consumption data were recorded during the whole procedure. At the end of the experiment, radioimmunity assays were used to measure the levels of serum cortisol. ResultsRats exposed to CUMS showed reduced water consumption; a preference for sucrose solutions; and decreased squares crossed, less rearing and in