成人膳食类胡萝卜素摄入量估算值与HPLC测定值的比较_医学论文.docVIP

成人膳食类胡萝卜素摄入量估算值与HPLC测定值的比较_医学论文 成人膳食类胡萝卜素摄入量估算值与HPLC测定值的比较_医学论文 【摘要】 目的 评价采用美国类胡萝卜素成分数据估算的膳食类胡萝卜素摄入量水平与HPLC测定的膳食类胡萝卜素摄入量数据之间的差异和相关性。方法 采用双饭份法和称质量记录法，在夏、秋、冬、春4个季节，调查不同社区184名30～60岁居民连续3 d膳食，利用美国的食物成分数据建立β胡萝卜素、α胡萝卜素、β隐黄质、番茄红素、叶黄素玉米黄质5种类胡萝卜素的食物成分表，计算5种类胡萝卜素每日摄入量。同时，采用HPLC测定膳食样品中类胡萝卜素的含量。结果 用两种方法获得的夏、秋、冬、春4个季节的膳食中β胡萝卜素、α胡萝卜素、番茄红素摄入量均无明显差异(t=0.17～1.68,Pgt0.05)。除了秋季，夏、冬、春季β隐黄质的摄入量差异明显(t=2.35～8.51,0.05)。4个季节中，两组叶黄素玉米黄质摄入量水平均有明显差异(t=3.24～3.93,0.05)。两组5种类胡萝卜素的测定水平均存在显著的相关关系(r=0.45～1.00,0.05)。结论 以美国食物成分数据估算的中国居民膳食类胡萝卜素摄入量与HPLC测定值非常接近。表明在尚未建立中国食物的类胡萝卜素成分数据的情况下，借用美国食物成分数据估算中国居民膳食类胡萝卜素摄入量是比较可靠的。 【关键词】 类胡萝卜素 摄入量 膳食调查 HPLC测定 ［ABSTRACT］ObjectiveTo evaluate the accuracy of the estimated and calculated dietary carotenoid intakes of adults with the US carotenoid database.MethodsThis study consisted of 184 subjects, age 30-60 years, (84 males and 100 females) from various communities for a dietary investigation, in which the diets of continuous three days were recorded in summer, autumn, winter and spring in a year. The dietary records were used to calculate intakes of carotenoids (βcarotene, αcarotene, βcryptoxanthin, lycopene, and lutein/zeaxanthin), based on American carotenoid database. Meanwhile, the 3day dietary samples from the dietary investigation were analyzed by HPLC for carotenoid contents, and analyzed values of dietary carotenoid intakes were thus obtained. Two kinds of carotenoid intake values were compared statistically.ResultsThere was no significant difference between calculated and analyzed values of βcarotene, αcarotene and lycopene intakes in the four seasons during a year (t=0.17-1.68,Pgt0.05). The two kinds of intake values of βcryptoxanthin in summer, winter, spring were different significantly (t=2.35-8.51,0.05). For intakes of lutein/zeaxanthin, the differences of two kinds of values existed in every season of the year (t=3.24-3.93,0.05). The intake values from calculation and HPLC analysis were correlated on all five carotenoids and in the four season