放射性125I粒子照射小鼠膀胱癌模型的实验研究_药学论文.docVIP

放射性125I粒子照射小鼠膀胱癌模型的实验研究_药学论文 放射性125I粒子照射小鼠膀胱癌模型的实验研究_药学论文 作者： 杜明华，高立宁，姚茂银，郭金和 【关键词】 碘 【Abstract】 AIM: To get insights into the biological effect of implanting 125I seeds to treat bladder cancer model of T139 mice and to provide an experimental basis for clinical utilization of 125I seed brachytherapy for bladder cancer. METHODS: Eight mice models bearing T139 bladder cancer were divided into 2 groups (experimental, 4; control, 4) at random. 125I seeds were implanted into the tumor in experimental group while the empty seed was implanted in the control group at the same position as the experimental group. The experimental mice were executed after 24 d. Three tissues were cut from different positions according to the different distance (0.6, 1.2, 1.8 cm) from the implanted 125I seeds and the tumor cells were primarily cultured and the pathomorphological changes, growth and proliferation of tumor cells were dynamically recorded. Tumor cell count, efficiency and inhibitory rate of colony formation were calculated. RESULTS: ① The cell number of experimental group was obviously fewer than that of control group at the same time point. There was a significant difference in the number of cultured cells from different positions of experimental group. ② The inhibitory rate of colony formation was 41.84%. ③ Variance analysis of total cloning efficiency of the cells from different positions showed a significant difference between experimental group and control group (0.01), and among different positions of experimental group (0.01). CONCLUSION: The γ radiation from implanted 125I seeds directly destroys tumor cells within the effective range. The radiation dose and killing effect of γray decreased with the increasing distance from 125I seeds. 【Keywords】 125I seed; bladder neoplasms; interstitial implant; mice 【摘要】 目的： 揭示125I粒子照射膀胱癌的生物学效应，为临床应用125I粒子治疗膀胱癌提供实验依据. 方法： 把T739近交系小鼠膀胱低分化移行细胞癌动物8只随机分为两组，实验组和对照组，每组4只. 在小鼠瘤体内植入MSI125型125I粒子，在对照组小鼠瘤体内相应部位植入粒子空壳，继续饲养24 d后处死. 在距粒子植入部位0.6,