板蓝根对机体免疫功能及流感病毒FM1的作用_药学论文.docVIP

板蓝根对机体免疫功能及流感病毒FM1的作用_药学论文 板蓝根对机体免疫功能及流感病毒FM1的作用_药学论文 作者：金明哲， 任东鲜，孟繁平，李晓眠 【关键词】 板蓝根；,,流感病毒；,,淋巴细胞；,,红细胞免疫黏附；,,抗病毒作用 　　摘要：目的研究板蓝根提取物对小鼠机体免疫功能的影响及其抗流感病毒(FM1)的作用。方法昆明种小鼠腹腔注射板蓝根提取液，检测红细胞免疫黏附功能等各项免疫指标;昆明种小鼠滴鼻感染流感病毒FM1后腹腔注射板蓝根提取液，观察板蓝根提取液的抗病毒作用。结果与正常对照组相比，板蓝根组脾指数增加（P ＜0.05）；T、B淋巴细胞刺激指数、RBCC3bR花环阳性率都明显增高，RBCIC花环阳性率显著降低，有显著性差异（P＜0.01）。与阴性对照组相比，板蓝根组肺指数降低（P＜0.05），体重增加值高（P ＜0.05)。结论 板蓝根提取物具有很强的增强机体免疫功能的作用，其提高红细胞免疫系统功能的作用极其显著；同时板蓝根提取物也有一定的抗流感病毒作用。 　　关键词：板蓝根； 流感病毒； 淋巴细胞； 红细胞免疫黏附； 抗病毒作用 　　Abstract：ObjectiveTo study the strengthening function of extract of Radix Isatidis on immune system in Kunming mice and its antiviral effects on influenza virus (FM1). MethodsExtract of Radix isatidis was intraperitoneally injected to Kunming mice. Lymphocyte proliferation assay and rosette formation test were used to measure the cellular immune function and humoral immune function and erythrocytic immune adhesive function of mice. FM1 virus was dropped nasally in Kunming mice, then the animals were control intraperitoneally injected extract of Radix isatidis, the antiviral effects of Radix isatidis were observed.ResultsCompared with control group,spleen index in the Radix Isatidis group increased（F=4.578, P ＜0.05）. Compared with control group, SI of T lymphocyte in the Radix Isatidis group increased significantly（F=15.537, P ＜0.01）, SI of B lymphocyte increased obviously（F=11.050, P ＜0.01）, and the rate of RBCC3bR was increased（F=7.865, P ＜0.01）, too. Compared with control group the rate of RBCIC in the Radix Isatidis group decreased greatly（F=20.354, P ＜0.01）. Compared with negative group, Lung index in the Radix Isatidis group decreased（F=47.020, P ＜0.05）. The degree of change in weight of that group was higher than negative group（F=25.877, P ＜0.05）. ConclusionRadix Isatidis has strong strengthening function on immune system in Kunming mice, its enhancing function on red cell immune adherence (RCIA) is extremely significant. Moreover，it also has antiviral effects on influenza virus. 　　Key wo