毁损海洛因依赖大鼠伏隔核对戒断症状及血浆β-内啡肽的影响_医学论文.docVIP

毁损海洛因依赖大鼠伏隔核对戒断症状及血浆β-内啡肽的影响_医学论文 毁损海洛因依赖大鼠伏隔核对戒断症状及血浆β-内啡肽的影响_医学论文 【摘要】 目的 观察毁损海洛因依赖大鼠伏隔核(NAc )对戒断症状及血浆β-内啡肽（β-EP）的影响。方法 ①海洛因依赖组（实验组）按逐日递增原则皮下注射海洛因建立海洛因依赖模型，首日每次剂量3 mg/kg，雌雄不拘，每日2次，连续9天皮下注射海洛因；对照组注射等量生理盐水。②毁损NAc：毁损电极插入NAc，通直流电进行毁损。实验组大鼠20只，分为实验Ⅰ组（海洛因依赖组）和实验Ⅱ组（依赖后行NAc毁损），每组10只；对照组大鼠20只分为对照Ⅰ组（NAc未毁损组），对照Ⅱ组（NAc毁损组），每组10只。比较实验Ⅰ组和实验Ⅱ组戒断症状的变化；③β-EP检测：实验Ⅰ组、实验Ⅱ组、对照Ⅰ组、对照Ⅱ组大鼠取躯干血置入抗凝试管中，混匀离心，放射免疫法测定4组大鼠血浆β-EP水平,比较各组大鼠血浆β-EP水平的变化。结果 ①戒断症状：毁损大鼠NAc后，除湿抖症状外，其他戒断症状均较毁损前减轻（0.01）；②血浆β- EP：与对照Ⅰ组比较，实验Ⅰ组血浆β- EP水平下降，对照Ⅱ组血浆β- EP水平上升（P均lt0.05）；实验Ⅱ组血浆β- EP水平上升，与实验Ⅰ组比较差异有显著性（0.05）。 结论 毁损NAc后，海洛因依赖大鼠的戒断症状减轻NAc参与β- EP水平的调节。 【关键词】 海洛因依赖 伏隔核 β-内啡肽 Abstract: Objective To observe the changes in withdrawal signs and plasma β-EP after ablating the NAc in heroin-dependent rats. Methods SD rats were divided into four groups of 10. TestⅠ and testⅡ groups were made addicts by starting with heroin 3 mg/kg hypo bid and increasing the dosage every day for a total of 9 days, while the controlⅠ and Ⅱ groups were given equivalent volumes of NS. Electric ablation of the NAc nucleus was performed in testⅡ and controlⅡ groups. The withdrawal signs were compared between the two test groups following the naloxone therapy. Finally， blood samples were taken to determine the level of β-EP for comparison between the four groups. Results The withdrawal signs were less marked ｉｎ　ｔｅｓｔⅡ　ｇｒｏｕｐ　ｔｈａｎ　ｉｎ　ｔｅｓｔⅠ　ｇｒｏｕｐ（ａｆｔｅｒ　ＮＡｃ　ａｂｌａｔｉｏｎ，　0.05）. The level of β－ＥＰ ｉｎ blood plasma was found ｌｏｗｅｒ　ｉｎ　ｔｅｓｔⅠ　ｇｒｏｕｐ　ｔｈａｎ　ｉｎ　ｃｏｎｔｒｏｌⅠ　ｇｒｏｕｐ　（ａｆｔｅｒ　ａｄｄｉｃｔｉｏｎ，　0.05）,　ａｎｄ　ｈｉｇｈｅｒ　ｉｎ　ｃｏｎｔｒｏｌⅡ　ａｎｄ　ｔｅｓｔⅡ　ｇｒｏｕｏｐｓ　（ａｆｔｅｒ　ＮＡｃ　ａｂｌａｔｉｏｎ，0.05）. Conclusion After the NAc ｉs destroyed, the withdrawal signs are alleviated.　The NAc participateｓ in　ｔｈｅ regulation of β-EP. Ｋｅｙ　ｗｏｒｄｓ： ｈeroin dependence； ｎucleus accumbens； β-EP 大量研究资料表明，中脑边缘多巴胺系统是一切药物成瘾的心理依赖基础［1］。中脑边缘多巴胺系统包括多巴胺(dopamine,DA )神经元集中的中脑腹侧背盖区（ventraltegmental area, VTA）及其主要投射区伏隔核(nucleus accumbens,NAc)和额叶皮质(prefrontal contex,PFC)等边缘前脑区域。NAc是产生奖赏效应的关键核团,在阿片类药物依赖形成机制中起着重要作用。然而，有关NAc毁损后戒断症状报