氧化苦参碱在大鼠肾间质纤维化进程中的保护作用_医学论文.docVIP

氧化苦参碱在大鼠肾间质纤维化进程中的保护作用_医学论文 氧化苦参碱在大鼠肾间质纤维化进程中的保护作用_医学论文 作者：姚钢炼，宁宁，高登峰，桂保松 【关键词】 氧化苦参碱；纤维化；肾间质；转化生长因子 　　摘要：目的 探讨氧化苦参碱对大鼠肾间质纤维化进程的干预作用，并阐明其部分机制。方法 40只雄性SD大鼠，随机分为假手术组、模型组、苯那普利组、氧化苦参碱大剂量组和小剂量组。以单侧输尿管结扎术建立输尿管梗阻的动物模型。术后第14天取术侧肾组织行HE、Masson染色及转化生长因子β1(TGFβ1)、α平滑肌肌动蛋白(αSMA)和Ⅲ型胶原(ColⅢ)免疫组化检测，并运用图像分析系统做半定量分析。结果 经氧化苦参碱干预后，梗阻侧肾脏的αSMA 、TGFβ1和ColⅢ的表达均显著低于模型组(0.01)；大剂量氧化苦参碱组均低于苯那普利组(0.05)；氧化苦参碱大剂量组和小剂量组之间无显著性差异(Pgt0.05)。结论 氧化苦参碱可能是通过降低TGFβ1等细胞因子的表达而减少细胞外基质(ECM)产生细胞的活化，从而减少ECM的沉积，达到抗肾间质纤维化的作用。 　　关键词：氧化苦参碱；纤维化；肾间质；转化生长因子β；肌成纤维细胞 　　The protective function of oxymatrine on renal　interstitial fibrosis in rats 　　ABSTRACT: Objective To investigate the protective function of oxymatrine on the renal intestitial fibrosis in rats. Methods Forty male Sprague Dawley rats were randomly divided into 5 groups: sham group, model group, Lotensin group, largedose oxymatrine group, and smalldose oxymatrine group. The models were established by unilateral ureteral obstruction(UUO) of the left side. On the 14th day after operation, the obstructed kidney was taken out. Then, HE, Massion, and immunohistochemistry staining of transforming growth factorβ1(TGFβ1), αsmooth muscle acting (αSMA) and collagen Ⅲ were conducted. After that, semiquantitative analysis was performed. Results After oxymatrine, treatment, the expressions of αSMA, TGFβ1 and ColⅢ of the obstructed kidney in the treatment group were significantly lower than those in the model group (0.01) the expressions in the largedose oxymatrine group were lower than those in the lotensin group while there were no significant difference between the largedose and smalldose oxymatrine groups (Pgt0.05). Conclusion Oxymatrine may reduce the expression of cytokines such as TGFβ1, then the activation of cell producing ECM and then the sendimentation of ECM, thus preventing renal interstitial fibrosis. 　　KEY WORDS: oxymatrine fibrosis renal interstitium transforming growth factorβ myofibroblast 　　肾间质纤维化几乎是所有慢性肾脏疾病发展的最终结果，是导致慢性肾衰竭的主要原因之一[1]。肾小管间质细胞表型转化、致纤维化细胞