水飞蓟素对肾小管间质纤维化大鼠_医学论文.docVIP

水飞蓟素对肾小管间质纤维化大鼠_医学论文 水飞蓟素对肾小管间质纤维化大鼠_医学论文 【摘要】 目的 探讨水飞蓟素对肾小管间质纤维化大鼠小管上皮-间质转分化的干预作用。方法 利用单侧输尿管梗阻诱导建立肾小管间质纤维化大鼠模型，实验SD大鼠随机分为梗阻组24只、治疗组24只、假手术组8只，梗阻组和治疗组分别于实验第7天、14天、21天各收获动物8只，假手术组于第21天一次处死动物，进行肾组织病理学观察，利用免疫组织化学对转化生长因子-β1(TGF-β1）和α-平滑肌肌动蛋白（α-SMA）在肾小管间质纤维化大鼠中的表达变化进行动态观察及水飞蓟素的干预影响。结果 实验第7天梗阻组大鼠即表现出小管损伤和炎症细胞浸润（P lt0.05）,随病程进展，上述病变呈进行性加重（P lt0.05），水飞蓟素治疗组动物在各时间点组织学的改善同梗阻组相比有明显减轻（P lt0.05）。免疫组织化学显示水飞蓟素在各观察时间点能显著下调TGF-β1和α-SMA在肾小管间质中的表达（P lt0.01）。结论 水飞蓟素通过下调TGF-β1的表达，有效干预上皮-间质转分化达到改善肾脏纤维化的作用。 【关键词】 水飞蓟素 肾小管间质纤维化 肾小管上皮-间质转分化 转化生长因子-β1 【Abstract】 Objective To explore the effect of silymarin on epithelial-mesenchymal transition (EMT) in rats with tubulointerstitial fibrosis induced unilateral ureteral obstruction. Methods 56 Sprague-Dawley rats were randomly divided into three groups: operated group(n=24), silymarin treated group（n=24）and sham-operated group(n=8).Renal tubulointerstitial fibrosis model was established via unilateral ureteral obstruction(UUO). In operated group and treated group, eight rats were killed at the 7d, 14d and 21d after operation to examine the histological changes of renal tissue, but all 8 rats in sham-operated group were only killed at 21d. Histological changes were observed in tubulointerstitial injury under microscope.The expression of transforming growth factor- beta1（TGF-β1）and α-smooth muscle action(α-SMA) in renal tissue were determined with immunohistochemical method, respectively. Results Renal interstitium injury was seen in operated group and treated group at day 7,and became aggravating after day 7. The degree of renal tubulointerstitial fibrosis in rats from treated group was lighter than that of operated group at day 7, 14, 21(P lt0.05). Compared with sham-operated group, the expression of TGF-beta1 and α-SMA were significantly up-regulated at day 7,14,21in operated group and treated group (all P lt 0.05), After intervention with silymarin the up-regulating above mentioned parameters were all significantly inhibited (all P lt 0.05). C