气阴双补法对干燥综合征小鼠的实验研究_医学论文.docVIP

气阴双补法对干燥综合征小鼠的实验研究_医学论文 气阴双补法对干燥综合征小鼠的实验研究_医学论文 【摘要】 目的研究气阴双补法对干燥综合征小鼠的作用。方法将60只Bacb/C小鼠，按体质量随机分为6组，每组10只，即正常组，模型组，羟氯喹组，增液布津汤小剂量组、中剂量组和大剂量组。对照组用羟氯喹片剂，观察体质量、摄食量、饮水量、唾液流量、脾指数及颌下腺病理形态学变化。结果增液布津汤能有效缓解口干、口腔烧灼感、眼干、眼异物感、鼻干等临床症状。实验室检查方面能增加唾液流量及泪流量，病理显示能抑制淋巴细胞浸润。结论运用气阴双补不仅能缓解干燥综合征小鼠临床症状，而且能纠正免疫紊乱,疗效优于对照组。 【关键词】 气阴双补； 干燥综合征； 小鼠 　　Abstract：ObjectiveTo research the function of the method of nourishing both qi and blood on the mice having SS.MethodsPut the 60 Bacb/C mice into 6 groups according to their weight at random, there were 10 mice in each group.The groups included the normal group, the model group, the hydroxychloroquine group, the low, middle and high dose group using Zengye Bujin Decoction. We used hydroxychloroquine tablets on the mice in the control group and then observed the index weight, food intake, water intake, salivary flow rate, spleen index and the pathomorphology observation of submandibular gland.ResultsZengye Bujin Decoction could effectively relieve dry mouth, burning mouth sensation, dry eye, eye foreign body sensation, dry nose and some other clinical symptoms. The examination of lab showed it could increase salivary flow rate and tear flow rate. Pathology also showed it could restrain the infiltration of lymphocyte.ConclusionThe use of the method of nourishing both qi and blood can not only relieve the clinical symptoms of the mice having SS, but also correct immunologic derangement, whose efficacy is better than those in the control group. 　　Key words：Nourishing both qi and blood ； SS； Mice； Experimental research 干燥综合征(Sjogren′s syndrome，SS)是一种侵犯外分泌腺体（尤以唾液腺和泪腺为主）的慢性自身免疫病。本病病因可能与遗传、免疫、性激素、内分泌和病毒感染等因素有关。中医学无此病名，根据其发病及临床特征，将之归属祖国医学“燥证”“燥痹”范畴。金元时期，刘河间在《素问玄机原病式·燥类》中指出：“诸涩枯涸，干则皴揭，皆属于燥”；并创立麦门冬饮子以治之。明清以后，各家对燥证已论述颇多，均从不同角度对燥证进行了较系统的探讨和研究。最具代表性的是清代喻嘉言，认为除燥伤于外皮肤皴揭之外，燥伤于内而致的精血枯涸亦属于燥证范畴，并根据刘河间“风热胜湿为燥”的理论提出了辛凉甘润法治疗燥证，代表方剂为著名的清燥救肺汤。然而，从临床研究发现，SS除与阴虚燥毒有关外，与气虚因素密切相关，运用气阴双补法对干燥综合征小鼠实验研究，通过研究其对小鼠的干预作用，旨在为临床治疗提供确切依据。 　　1 材料与仪器 　　1.1 动物12周龄Bacb/C小鼠，60只，18～22 g，雌性，由南京青