正常和6羟多巴胺毁损大鼠脚桥核神经元对胆碱能M受体刺激的反应_药学论文.docVIP

正常和6羟多巴胺毁损大鼠脚桥核神经元对胆碱能M受体刺激的反应_药学论文 正常和6羟多巴胺毁损大鼠脚桥核神经元对胆碱能M受体刺激的反应_药学论文 作者：李小颖，向莉，尤雪梅，惠艳娉，李立博，侯辰，张巧俊 【摘要】 目的 在体水平上观察选择性胆碱能M受体激动剂氧化震颤素(Oxo对正常和6羟基多巴胺(6毁损大鼠脚桥核(PPN)神经元自发电活动的影响。方法 采用玻璃微电极细胞外记录和PPN内注射Oxo，观察PPN神经元电活动的变化。结果 与正常组大鼠相比，6毁损组大鼠PPN神经元的平均放电频率增高(0.01)，神经元活动趋于不规则(0.001);PPN内注射Oxo对正常组和6毁损组大鼠的PPN神经元放电频率均可产生升高、降低和无变化三种改变。然而，PPN内注射Oxo对正常组大鼠PPN神经元的平均放电频率无明显影响，但Oxo使6毁损组大鼠PPN神经元的平均放电频率显著降低(0.001)。结论 胆碱能M受体激动剂抑制损毁组大鼠PPN神经元的活动，其作用机制是复杂的，涉及到PPN内突触前和突触后胆碱能受体。 【关键词】 脚桥核;M型胆碱能受体;氧化震颤素;帕金森病;电生理学 　　ABSTRACT: Objective To explore the effects of oxotremorine M (Oxof PPN neurons was observed by extracellular recording in vivo and local injection of OxoM in the PPN.Results The results showed that the mean firing rate of PPN neurons in the lesioned rats was significantly increased compared to that in normal rats (0.01), and that the firing pattern of the neurons changed toward a more irregular firing after the lesion (0.001). IntraPPN injection of OxoM induced increase, decrease or no change in the firing rate of PPN neurons in both the normal and 6OHDAlesioned rats. However, the mean firing rate of PPN neurons in normal rats remained unaltered by the stimulation of M receptors, but the mean firing rate of PPN neurons in the lesioned rats decreased significantly by intraPPN injection of OxoM (0.001).　Conclusion M receptor agonist inhibits the activity of the PPN neurons in lesioned rats; the action mechanism of the agonist is complicated, involving not only postsynaptic but also presynaptic M receptors located in different sites within the PPN. 　　KEY WORDS: pedunculopontine nucleus; M acetylcholine receptor; oxotremorine; Parkinsons disease; electrophysiology 　　脚桥核(pedunculopontine nucleus, PPN)是一中脑的神经结构，由胆碱能和非胆碱能神经元组成[1]。解剖学和电生理学研究表明PPN与基底神经节有丰富的交互纤维联系，在运动调控中起重要作用[1]。PPN的主要传入纤维包括来自黑质网状部(substantia nigra pars reticulate, SNr)和脚内核(endopeduncular nucleus, EP)的γ氨基丁酸能神经纤维，以及来自底丘脑核(subthalamic nucleus, STN)和额叶皮质的谷氨酸能神经纤维[1。PPN的上行传出纤维主要投射到黑质致密部(substantia nigra pars compact, SNc)、S