桥本甲状腺炎中Bcl┐2和Bax与PCNA关系的免疫组化双标法_医学论文.docVIP

桥本甲状腺炎中Bcl┐2和Bax与PCNA关系的免疫组化双标法_医学论文 桥本甲状腺炎中Bcl┐2和Bax与PCNA关系的免疫组化双标法_医学论文 作者：姬秋和　宋民喜　张雅萍　张南雁　马福成　杨守京 【关键词】 桥本甲状腺炎 关键词: 桥本甲状腺炎细胞凋亡增殖增殖细胞核抗原 摘 要:目的 观察Bcl-2和Bax与PCNA在桥本甲状腺炎（HT）中表达及分布的关系. 方法 以16例毒性甲状腺肿（NTG）为对照，采用TUNEL及免疫组织化学双标记法分别检测16例HT患者甲状腺标本中的细胞凋亡水平及PCNA与Bcl-2和Bax的分布. 结果 凋亡率（AR）在HT组为（34.0±14.7）%，NTG组为（0.9±0.6）%增殖率（PR）分别为（8.5±3.6）%，（4.2±2.4）%AR/PR比值各为4.5±2.4和0.5±0.9.显示AR，PR及AR/PR比值在HT组均显著高于NTG组（0.01）.在HT中，Bcl-2，Bax免疫染色阳性率分别为88%和82%共表达细胞占Bcl-2或Bax阳性细胞的比例各为33%和16%. 结论 HT中甲状腺滤泡细胞凋亡与增殖活动均活跃，凋亡水平增高更为显著浸润淋巴细胞增殖活跃，凋亡少见.部分甲状腺滤泡细胞PCNA与Bcl-2或Bax共表达.其凋亡和增殖的失平衡可能与调节蛋白Bcl-2或Bax的作用有关，也可能是HT病理改变的主要机制之一. 　　Abstract:AIM To investigate relationship between the expression and distribution of PCNA and those of Bcl-2or Bax in Hashimoto’s thyroiditis（HT）.METHODS Specimen from nontoxic goiter（NTG，n=16）were used as the con-trol.Apoptotic level and expressions as well as distributions of PCNA，Bcl-2and Bax in thyroid tissues from16HT pa-tients were detected by TUNEL and immunohistochemical double labelled staining repectively.RESULTS Apoptotic rates（AR）in the HT and NTG groups were（34.0±14.7）%and（0.9±0.6）%respectively，while the prolifertive rates（PR）were（8.5±3.6）%and（4.2±2.4）%，and AR/PR ra-tios in HT and NTG were4.5±2.4，0.5±0.9respectively，which indicated that AR，PR，AR/PR ratio in HT were sig-nificantly higher than those in NTG（0.01）.The rates of positive immunostaining for Bcl-2and Bax on part of thyro-cytes from HT were88%and82%，and Bcl-2or Bax positive percentages of co-expression thyrocytes were33%and16%.CONCLUSION Both apoptotic and proliferative activities in thyrocytes of HT group were active，especially apoptotic lev-el，and there was a high proliferative activity in infiltrating lymphoid cells where apoptosis is rarely seen.There was co-expression of PCNA with Bcl-2or Bax on part of thyrocytes in HT.And both Bcl-2and Bax might regulate the imbalance between apoptosis and proliferation which may be the major mechanisms of pathologic changes in the HT group. 　　0 引言 　　桥本甲状腺炎（