栀子苷镇痛和抗炎作用的研究_药学论文.docVIP

栀子苷镇痛和抗炎作用的研究_药学论文 栀子苷镇痛和抗炎作用的研究_药学论文 作者：方尚玲，刘源才，张庆华，林英， 陈道玉，李世杰 【摘要】 　　目的研究栀子苷的镇痛和抗炎作用。方法小鼠热板实验，醋酸诱发小鼠扭体反应实验，栀子苷对二甲苯致小鼠耳肿胀实验，栀子苷对醋酸诱发小鼠毛细血管通透性实验，栀子苷对角叉菜胶致小鼠足肿胀实验等。结果栀子苷25 mg/kg剂量（P＜0.05）能延长热刺激所致小鼠痛觉反应时间，栀子苷50 mg/kg剂量组和12.5 mg/kg剂量组对醋酸诱发小鼠扭体反应有明显的抑制作用（P＜0.05），最大扭体次数减少百分率可达55%，具有一定的镇痛作用；栀子苷12.5 mg/kg剂量组（P＜0.05）和25 mg/kg剂量组（P＜0.01）对二甲苯致小鼠耳肿胀均有明显的抑制作用，栀子苷大剂量（50 mg/溶液kg）组能导致腹腔毛细血管渗透液的吸光值显著降低（P＜0.01），即对急性炎症渗出有较明显的抑制作用；栀子苷对角叉菜胶致小鼠足肿胀没有明显抗炎作用，炎症渗出液中PGE2的含量与对照组比较无明显差异，其抗炎作用可能与PGE2释放没有关系。结论 栀子苷有一定的镇痛和抗炎作用。 【关键词】 栀子苷 镇痛作用 抗炎作用 　　Abstract：ObjectiveTo study the analgesic and anti-inflammatory effects of geniposide. MethodsThe plate heating tests and the writhing response induced by acetic acid; ear swollen of mice induced by dimethy benzene; absorbance of peritioneal capillary extracasate; foot swollen in mice induced by Carrageenan were perfromed. ResultsAccording to the plate heating test, the dose of geniposide (25 mg/kg) could prolong the time of response for pain. The writhing response of mice were obviously inhibited （P＜0.05） in the group of geniposide (50 mg/kg) and the group (12.5 mg/kg), and the maximal decreased percentage of writhing achieved 55%, showing certain analgesia effect. The ear swollen of mice induced by dimethy benzene was obviously inhibited in the group （P＜0.05） of geniposide (12.5 mg/kg) and the group （P＜0.01） of geniposide (25 mg/kg). The high dose of geniposide (50 mg/kg) could lead to obvious decrease of absorbance of peritoneal capillary extravasate which meant obvious inhibiting effect on acute inflammatory extravasate. The geniposide did not show obvious anti-inflammatory effect on foot swollen in mice induced by Carrageenan, and there was no clear difference between the concentration of PGE2 in inflammatory extravasate and the contrast. And maybe there is no relationship between the anti-inflammatory effect of geniposide and the release of PGE2.ConclusionGeniposide has certain analgesic effect and anti-inflammatory effect. 　　Key words：Geniposid