桥本甲状腺炎中TRAIL及其死亡受体DR4，DR5的表达_医学论文.docVIP

桥本甲状腺炎中TRAIL及其死亡受体DR4，DR5的表达_医学论文 桥本甲状腺炎中TRAIL及其死亡受体DR4，DR5的表达_医学论文 作者：付建芳　姬秋和　黄威权　张南雁 【关键词】 肿瘤坏死因子 关键词: 脱噬作用肿瘤坏死因子配体受体，甲状腺炎，自身免疫性 摘 要:目的 研究肿瘤坏死因子相关凋亡诱导配体（TRAIL）及其死亡受体（DR4，DR5）在桥本甲状腺炎发病机制及病理改变中的作用及意义. 方法 采用免疫组织化学方法和图像分析，检测18例桥本甲状腺炎患者甲状腺标本中TRAIL，DR4和DR5的表达及分布. 结果 桥本甲状腺炎组甲状腺滤泡细胞TRAIL，DR4和DR5免疫反应产物的相对含量分别为（0.095±0.018），（0.080±0.020），（0.084±0.015）结节性甲状腺肿组甲状腺滤泡细胞TRAIL、DR4和DR5免疫反应产物的相对含量分别为（0.045±0.014），（0.041±0.020），（0.042±0.016）.桥本甲状腺炎组以上3种免疫反应产物的相对含量均显著高于结节性甲状腺肿组（0.05）.桥本甲状腺炎病理改变越重，甲状腺滤泡细胞TRAIL，DR4和DR5免疫染色越强浸润淋巴细胞中TRAIL，DR4和DR5免疫染色较弱或呈阴性. 结论 TRAIL，DR4和DR5在桥本甲状腺炎中的甲状腺滤泡呈特征性分布的高表达，病理改变越重，表达越强提示甲状腺滤泡细胞通过自身表达TRAIL，DR4和DR5，以细胞凋亡形式破坏甲状腺滤泡细胞. Keywords:apoptosistumor necrosis factorligandsrecep-torsthyroiditis，autoimmune 　　Abstract:AIM To investigate the significence of the ex-pression of TNF-related apoptosis-inducing ligand（TRAIL），death receptor4（DR4）and death receptor5（DR5）in the pathogenesis and pathological changes in Hashimoto’s thy-roiditis（HT）.METHODS Expressions and distributions of TRAIL，DR4and DR5in thyroid tissues from18patients with HT were investigated by immunohistochemical and in situ quantification methods.RESULTS The relative amounts of positive immunostaining for TRAIL，DR4and DR5in thyrocytes from HT and NTG patients were（0.095±0.018）and（0.045±0.014），（0.080±0.020）and（0.041±0.020），（0.084±0.015）and（0.042±0.016），respectively.The relative amounts of positive immunostaining for TRAIL，DR4and DR5in thyrocytes from HT patients were siginifi-cantly greater than those from the nontoxic goiter（NTG）pa-tients（0.05）.Strongly positive immunostained thyro-cytes of HT for TRAIL，DR4and DR5were mainly distribut-ed in follicles adjancent to lymphocytic infiltrates.Staining of infiltrating lymphocytes for TRAIL，DR4and DR5was found to be rather weak or even negative.CONCLUSION High characteristic expressions of TRAIL，DR4and DR5in thyro-cytes from HT were observed.The immunostaining of infil-trating lymphocytes for TRAIL，DR4and DR5was rather weak or negative.Expressi