正常与不同证候 H22 荷瘤小鼠肾上腺高表达的基因_医学论文.docVIP

正常与不同证候 H22 荷瘤小鼠肾上腺高表达的基因_医学论文 正常与不同证候 H22 荷瘤小鼠肾上腺高表达的基因_医学论文 作者：潘志强 方肇勤 卢文丽 梁超 吴中华 刘小美 侯俐 张辉 卓少元 廖明娟 高必峰 【摘要】 目的：揭示正常与不同证候昆明种荷瘤小鼠肾上腺高表达的基因。方法：采用昆明种正常小鼠与腋下接种 H22腹水瘤细胞的小鼠，取接种肿瘤后第 8 天（早期）邪毒壅盛证与气虚证、第 21 天（中期）阳气虚证、第 29 天（中晚期）气阴阳虚证荷瘤小鼠与正常对照组小鼠肾上腺，抽提 RNA、纯化及 Gene Chip Mouse Exon 1.0 ST Array 检测。结果：筛选到402 条高表达的基因，其中有 1 个及以上组别芯片读数计算值大于 5 000 的有 88 条。这些基因主要参与代谢和基因表达的调控，部分参与化学合成、化学加工修饰、运输和信号转导，以及部分参与免疫、细胞周期和细胞凋亡、发育；另有许多基因功能未知。结论：这些在正常与疾病小鼠肾上腺内持续且稳定表达的基因，可能对于维持肾上腺的正常生理功能具有重要的生物学意义；鉴于气虚证荷瘤小鼠这些基因大多表达量低于正常小鼠和同期的邪毒壅盛证小鼠，反映出气虚证候的本质及特征；再次，4 个不同证候荷瘤小鼠肾上腺这些基因表达差异分别有集中的趋势，印证了中医学“同病异证”的理论及其本质，要求辨证论治。 【关键词】 肾上腺；小鼠；基因表达；基因芯片；肿瘤 〔Abstract〕 Objective To reveal the character of high expression genes in adrenal gland of normal and H22 tumour mice about typical symptoms and signs. Methods By the quantitative four diagnosis and syndrome differentiation methods and GeneChip Mouse Exon 1.0 ST Array, we observed adrenal gland gene expression about poisonous pathogenic factors syndrome and asthenia of qi syndrome on 8th day, asthenia of yang and qi syndrome on 21th day, asthenia of qi and yin and yang syndrome on 29th day with H22 tumour mice. Results 402 high expression genes were gained, and the computed value of 88 genes are more than 5 000. These genes participate in metabolism, gene expression and regulation, chemistry synthesis and processing and modify, transport, signal transduction, immunization, cell cycle, apoptosis, development,and some genes function are unknown. Conclusion Those genes are persistent and steady expression in normal and H22 tumour mice adrenal that could be important biology significance. Genes expressed down-regulate may be reflect the essence and feature of asthenia of qi syndrome. Otherwise, differentially expressed genes in adrenal gland have coherent tendency among four different symptoms and signs, which confirm the theory and essence about different symptoms and signs of the same disease, and it is essential to execute syndrome differentiation and treatment. 〔