氟伐他汀对博来霉素A5 致大鼠肺纤维化的治疗作用_医学论文.docVIP

氟伐他汀对博来霉素A5 致大鼠肺纤维化的治疗作用_医学论文 氟伐他汀对博来霉素A5 致大鼠肺纤维化的治疗作用_医学论文 作者：赵峰　戚好文　赵一玲　吕荣 【关键词】 肺纤维化 关键词: 肺纤维化普伐他丁博来霉素A5 摘 要:目的 观察氟伐他汀（fluvastatin）对博来霉素（bleomycinA5 ）所致的大鼠肺纤维化的治疗作用，为肺纤维化的治疗探索一种新的方法. 方法 雄性SD大鼠30只，随机分为正常对照组（C组）、博来霉素组（B组）及根据给博来霉素后第1，15日始每日按20mg kg-1 及10mg kg-1 给予氟伐他汀灌胃治疗分为4个治疗组（TH 1，TL 1，TH 15及TL 15组）.28d后处死所有动物，提取肺组织，HE染色，显微镜下观察，Leica Q500MC图像分析系统测定肺泡间隔成纤维细胞数量、厚度及肺间质面积.Masson、苦味酸天狼猩红染色，观察基质胶原及Ⅰ，Ⅲ型胶原变化.肺组织匀浆测定羟脯氨酸含量，并比较各组间变化. 结果 ①B组的肺泡间隔成纤维细胞数量、厚度、肺间质面积及肺组织羟脯氨酸含量均高于C组（0.01），各氟伐他汀治疗组的以上各观察指标均低于B组（0.01或0.05）.②肺泡间隔成纤维细胞数量、厚度、肺间质面积在TH 1组低于TH 15组（0.01），TL 1组低于TL 15组（0.01或0.05）.③B组基质胶原及Ⅰ，Ⅲ型胶原分布较C组明显增多，各氟伐他汀治疗组均有不同程度的减少. 结论 氟伐他汀对博来霉素所致的大鼠肺纤维化具有明显治疗作用，且早期治疗较晚期治疗效果更为明显. 　　Keywords:pulmonary fibrosispravastatinbleomycin A5 Abstract:AIM To investigate the therapeutic effect of flu-vastatin in bleomycin-induced pulmonary fibrosis of rats.METHODS Thirty SD rats were divided into six groups:The normal control group（Group C），the model group treat-ed with bleomycinA5 （Group B）and the rest（Groups TH 1，TH 15，TL 1and TL 15）so named according to the dose of flu-vastatin which were20mg kg-1 and10mg kg-1 and the date which were the first and the fifteenth day after the rats were given bleomycinA5 respectively.All the rats were killed on the twenty-eighth day.The number of fibroblast and the thickness of alveolar wall，the area of mesenchyma in lung tissue were measured by HE staining.The extracellular ma-trix andⅠ，Ⅲtype collagen in lung tissue were observed by Masson and sirius red staining and hydroxyproline was mea-sured in lung tissue homogenates.RESULTS The number of fibroblast and the thickness of lung alveolar wall，the area of mesenchyma and the content of hydroxyproline in lung tis-sue were significantly higher in Group B than in Group C（0.01）.The data in the treatment groups were lower than those in Group B（0.01or 0.05）.It was also found that the data of HE staining were lower in Group TH 1than in Group TH 15（0.01）and in Group TL 1than in Group TL 15（0.01or 0.05）.In a