治疗乙型肝炎新药肝龙胶囊的药效学初步研究_药学论文.docVIP

治疗乙型肝炎新药肝龙胶囊的药效学初步研究_药学论文 治疗乙型肝炎新药肝龙胶囊的药效学初步研究_药学论文 作者：杜一民， 陈鸿珊 ， 李树楠， 李壮， 李小兵， 张华明， 方春生 【关键词】 肝龙胶囊；,,鸭乙型肝炎病毒；,,鸭乙肝动物模型；,,实验性肝损伤；,,美洲大蠊 　　摘要：目的观察肝龙胶囊对鸭乙型肝炎病毒的抑制效果和对实验性肝损伤的保护作用。方法（1）以鸭乙型肝炎病毒（DHBV）静脉注射感染雏鸭为模型，于感染第7天后分组灌胃肝龙（GL）胶囊浸膏0.5，1.5，3.0 g#12539;kg1#12539;d1，阳性对照组给予无环鸟苷(ACV)或磷羧基甲酸钠(PFA)。采用斑点杂交方法检测鸭血清DHBVDNA，以病毒抑制率为疗效指标；（2）以昆明种小鼠腹腔注射CCl4肝损伤为模型，给予GL浸膏(200 mg#12539;kg1)。测定SGPT值，并取肝脏作病理形态学观察。结果（1）GL三个不同剂量组对DHBV均有不同程度的抑制作用，有一定的量效关系；（2）GL降低CCl4肝损伤小鼠血清SGPT值并减轻肝细胞损伤。结论肝龙胶囊可抑制雏鸭体内DHBVDNA的复制并能保护CCl4所致的小鼠肝损伤。 　　关键词：肝龙胶囊； 鸭乙型肝炎病毒； 鸭乙肝动物模型； 实验性肝损伤； 美洲大蠊 　　The Hepatic Pharmacological Effects of Ganlong Capsules in vivo. 　　Abstract：ObjectiveTo observe the antiviral activity of Ganlong Capsule against duck Hepatitis B virus( DHBV) and its protective effects on experimental liver injury in vivo.Methods(1) Experimental researches on antiviral activity of Ganlong Capsules against duck hepatitis B virus: The duck Hepatitis B model was made by injecting DHBV into the veins of one-day-old Beijing duck on the seventh day after infected by DHBV, Ganlong Capsule was given by intragastric administration for 10 days to group I (0.5 g#12539;kg1#12539;d1), group II(1.5 g#12539;kg1#12539;d1), group III(3.0 g#12539;kg1#12539;d1), bid×10 d, and detected the levels of DHBV-DNA in the duck blood sera obtained separately on the day before giving Ganlong Capsule, the fifth day and tenth day after giving Ganlong Capsule, and the third day after withdrawing Ganlong Capsule, by serum dotblot hybridization. The trial was compared with acyclovor(ACV) or phosphonoformate(PFA). (2) Protective effects of Ganlong capsule on experimental liver injury in mice: Acute hepatic injury was induced by intraperitoneal injection of 0.2% CCl4 10 ml#12539;kg1, Ganlong(200 mg#12539;kg1) was given by intragastric or intraperitoneal administration for 2 days, bid. The serum SGPT were examined and the histopathological changes of hepatic tissue was measured.Results(1)The results of three experiments showed that the levels