消癌平对Ec-9706食管癌细胞的作用及机制实验研究_药学论文.docVIP

消癌平对Ec-9706食管癌细胞的作用及机制实验研究_药学论文 消癌平对Ec-9706食管癌细胞的作用及机制实验研究_药学论文 作者：张明智，何振，吴广银，邢舴，宋敏，王瑞林 【摘要】 　　目的探讨消癌平注射液对人食管癌细胞(Ec-9706)的抑制作用及其作用机理。方法①观察不同浓度消癌平注射液对Ec-9706的抑制率。②用倒置显微镜，丫啶橙染色（荧光显微镜）观察细胞用药前后形态变化；流式细胞仪检测用药前后细胞周期变化；免疫细胞化学法检测CyclinD1表达。结果①消癌平40，20 ，10，5 mg/ml浓度时对EC-9706细胞的生长抑制率分别为（90.15±2.74）%，（68.02±3.56）%，（48.73±1.79）%，（22.52±2.08）%。其OD值与空白对照组差异有统计学意义(0.05)。其IC50值为(10.89±1.05）mg/ml。②消癌平可诱导Ec-9706凋亡。③EC-9706经药物作用后，G0/G1期细胞百分比明显升高，S期细胞百分比明显下降，细胞被阻滞于G0/G1期。④Ec-9706在蛋白水平不表达CyclinD1。结论消癌平对Ec-9706细胞有较好的抑制作用。 【关键词】 消癌平； 食管癌细胞； 丫啶橙； 流式细胞术 　　Abstract:ObjectiveTo observe the inhibitory effect and mechanism of Xiaoaiping(XAP, a kind of traditional Chinese medicine) Injection on esophagus carcinoma cell line (Ec-9706). Methods①In vitro inhibition experiments were undertaken for Ec-9706 cells with XAP 40，20，10 and 5 mg/ml concentrations, respectively. ②The cellular shape was observed by invert microscope and acridine orange staining with fluorescent microscope, flow cytometry was applied to detect the difference of cell cycle, and immunohistochemistry was applied to the expression of CyclinD1. Results①XAP Injection could remarkably inhibit the growth of EC-9706 cells. When 40mg/ml, 20mg/ml, 10mg/ml, 5mg/ml XAP Injection was cultivated with EC-9706 cells, the IR was （90.15±2.74）%, （68.02±3.56）%, （48.73±1.79）%, （22.52±2.08）% respectively. The difference between every experimental group and control group was significant (P＜0.01). IC50 was （10.89±1.05）mg/ml. ②XAP could induce Ec-9706 cell line to apoptosis. ③Flow cytometry analysis showed that cellular periods were （59.56 ±1.73）%, （86.68±2.57）%, （83.26±1.86）% in G0/G1 period, respectively, after XAP Injection (10，20，40 mg/ml) mixed with Ec-9706 cellular strains for 48 hours . The differences between experimental group and control group were significant (0.05). ④CyclinD1 didnt express in protein level in Ec-9706 cell line. ConclusionXAP appears to have good inhibitory effect on esophagus carcinoma cellular strains. 　　Key words:Xiaoaip