环氧化酶-2在肝细胞癌及癌旁肝硬化中表达及意义_医学论文.docVIP

环氧化酶-2在肝细胞癌及癌旁肝硬化中表达及意义_医学论文 环氧化酶-2在肝细胞癌及癌旁肝硬化中表达及意义_医学论文 【摘要】 ［目的］ 探讨环氧化酶2（COX-2）在肝细胞癌（HCC）、癌旁肝硬化和正常肝组织中的表达及其意义。［方法］ 应用免疫组织化学法检测30例HCC石蜡包埋组织、25例癌旁肝硬化组织及10例正常肝组织标本中COX-2的表达情况，分析其与肝细胞癌临床病理特征间的联系。［结果］ COX-2蛋白在HCC、癌旁肝硬化中表达阳性率分别为70.0%和92.0%，显著高于在正常肝组织中的20.0%（P＜0.05），而COX-2在癌旁肝硬化组织中的表达高于HCC组织。COX-2蛋白在分化好HCC中的表达显著高于分化差的HCC（P＜0.05），转移组中COX-2蛋白表达显著高于无转移组（P＜0.01）；而COX-2蛋白的表达与肿瘤大小、有无包膜及AFP水平无关。［结论］ COX-2蛋白在肝细胞癌、癌旁肝硬化组织中高表达，表明COX-2可能在肝细胞癌的发生、发展过程中起着重要作用。 【关键词】 肝肿瘤 Expressions of Cyclooxygenase-2 in Hepatocellular Carcinoma Tissue and Carcinoma Adjacent Tissue with Cirrhosis Abstract: ［Purpose］ To investigate the expressions and significance of cyclooxygenase-2(COX-2) in hepatocellular carcinoma tissue, carcinoma adjacent tissues with cirrhosis and normal liver tissue. ［Methods］ The expression of COX-2 in 30 case with hepatocellular carcinoma, 25 cases with carcinoma adjacent tissues with cirrhosis and 10 cases with normal liver tissues were detected by SP immunohistochemical staining. The relationship between the expression and clinicpathologic features in HCC was analyzed. ［Results］ The expression of COX-2 in hepatocellular carcinoma and its adjacent tissues with cirrhosis were 70.0% and 92.0% respectively, and they were higher than that in normal liver tissue (20.0%). The COX-2 expression in carcinoma adjacent tissues with cirrhosis was higher than that in HCC. Expression of COX-2 in well differential HCC was higher than that in poor differential HCC, and higher in case with metastasis than that without metastasis(P＜0.01).The expression of COX-2 in HCC did not relate to tumor size, tumor capsula and the level of AFP. ［Conclusion］ High expression of COX-2 in HCC and carcinoma adjacent tissues with cirrhosis indicates that the COX-2 expression might play an important role in carcinogenesis and progression of HCC. Key words: hepatocellular neoplasms cyclooxygenase-2 immunohistochemistry 环氧化酶（cyclooxygenase，COX）是催化花生四烯酸（arachidonic acid，AA）合成内源性前列腺素（prostaglandin，PG）的限速酶。CO