熊胆冻干粉针剂对缺氧_药学论文.docVIP

熊胆冻干粉针剂对缺氧_药学论文 熊胆冻干粉针剂对缺氧_药学论文 作者：金光显， 任香善， 林贞花， 宋京郁， 玄延花， 金贤国 【关键词】 熊胆；,,血管内皮；,,丙二醛；,,一氧化氮 　　摘要：目的探讨熊胆冻干粉针剂对缺氧-再复氧损伤的血管内皮细胞的保护作用及抗动脉粥样硬化机制。方法采用脐静脉血管内皮细胞（human umbilical vein endothelial cell，HUVEC）体外原代培养方法。通过倒置相差显微镜观察和比较，各组内皮细胞形态，用MTT法检测吸光度值，利用比色法测定细胞上清夜中的丙二醛（malondialdehyde，MDA）和一氧化氮（nitric oxide ，NO）含量。结果 MTT法结果显示：正常组、熊胆组与缺氧－再复氧组比较有显著差异（0.01）。缺氧-再复氧组的MDA含量增高，而NO含量降低，正常组和熊胆组中检测结果相反。结论 熊胆冻干粉针剂对缺氧－再复氧损伤的血管内皮细胞有保护作用。 　　关键词：熊胆； 血管内皮； 丙二醛； 一氧化氮 　　Protective Effects of the Lyophiled Powder Injection of Bear Bile on Vascular Endothelial Cells Induced by Hypoxia-reoxygenation Injury 　　Abstract：ObjectiveTo study the protective effects of the lyophiled powder injection of bear bile on cultured vascular epithelial cells (VEC) induced by hypoxia-reoxygenation injury, and study the mechanisms of anti -atherosclerosis. MethodsBy using primary culture of human umbilical vein endothelial cells (HUVEC) in vitro, the VECs were divided into three groups.The morphological changes of VEC by the inverted microscope, the optical density (OD) value of cells were detected by MTT colorimetric method, content of MDA and NO in the supernatant fluid of cultured cells were detected by spectrophotometry.ResultsThe MTT assay showed that OD value had significant difference (0.01) between normal group, bear bile group and hypoxia-reoxygenation group. The MDA content increased and NO content decreased in the hypoxia-reoxygenation group, in contrast, MDA content decreased and NO content increased in the normal and bear bile groups. ConclusionThe results indicate that the lyophiled powder injection of bear bile has protective effects for the HUVEC injury induced by hypoxiareoxygenation injury . 　　Key words：Bear bile; Vascular epithelial cell; Malondiadehyde; Nitric oxide 　　缺血-再灌注是再灌注心肌损伤、缺血性脑卒中等严重疾病中常见的过程。缺血缺氧极易导致血管内皮细胞(vascular endothelial cell，VEC)的死亡和严重功能紊乱，从而进一步加重组织损伤。目前关于缺血-再灌注时细胞损伤的机制及对其的保护的研究主要集中在神经细胞和心肌细胞，对VEC的研究极少。本实验通过用连二亚硫酸钠造成缺氧－再复氧模型后用熊胆，观察其抗缺氧作用及其机制。 　　1 材料与方法 　　1.1 材料 　　1.1.1 人脐静脉内皮