清胰Ⅱ号对大鼠重症急性胰腺炎肺损伤的保护作用_医学论文.docVIP

清胰Ⅱ号对大鼠重症急性胰腺炎肺损伤的保护作用_医学论文 清胰Ⅱ号对大鼠重症急性胰腺炎肺损伤的保护作用_医学论文 【摘要】 　　目的：探讨大鼠重症急性胰腺炎(SAP)肺损伤与血清、肺组织中P选择素表达的关系及清胰Ⅱ号对肺损伤的保护作用. 方法：将30只Wistar大鼠随机分为对照组、SAP组和清胰Ⅱ号治疗组. 以40 g/L牛磺胆酸钠逆行注入胰胆管制作大鼠SAP并发急性肺损伤模型；造模1 h后将清胰Ⅱ号（6 mL/kg）用于模型，11 h后重复给药1次；造模24 h后取材，采用ELISA方法检测血清及肺组织中P选择素的表达，同时观察血清淀粉酶及胰、肺组织的病理改变. 结果： SAP组有胰、 肺组织损伤改变， 同时血清、 肺组织P选择素浓度由(8.6±1.0) ng/L， 11.42 (P25 11.07, P75 12.92) ng/L分别增高至(34.0±1.4) ng/L，72.47 (P25 57.78, P75 110.97) ng/L. 清胰Ⅱ号可降低血清、肺组织P选择素的浓度，减轻胰、肺组织的病理损伤程度. 结论：P选择素在SAP肺损伤发病机制中可能具有重要作用. 中药清胰Ⅱ号可能通过降低血清和肺组织的P选择素浓度减轻SAP时胰腺和肺组织病理学改变，从而发挥其保护作用. 【关键词】 重症急性胰腺炎；肺/损伤；大鼠；P选择素 　　【Abstract】 AIM: To study the correlation between the severe acute pancreatitis (SAP) complicated with acute lung injury and the expression of Pfect of Qingyi Ⅱ against the acute lung injury. METHODS: Thirty Wistar rats were randomly divided into control group (n=10), SAP group (n=10) and Qingyi Ⅱ treatment group (n=10). SAP models were established by injecting 40 g/L sodium taurocholate into the biliopancreatic duct. The Qingyi Ⅱ(6 mL/kg) was injected in the Qingyi Ⅱ group 1 and 12 h after the models were established. All the animals were sacrificed at 24 h. Then, the expression of Pselectin in serum and pulmonary tissue were assayed by ELISA, meanwhile, the pathological changes of pancreas and lung tissues were observed under microscope. RESULTS: The concentrations of Pselectin in serum and pulmonary tissue were lower in normal rats than in SAP rats (0.05), and the traditional Chinese medicine Qingyi Ⅱ reduced them significantly (0.05). Compared with SAP group, Qingyi Ⅱ group had less pathologic damage in both pancreatic and pulmonary tissues. CONCLUSION: Chinese medicine Qingyi Ⅱ redues the concentrations of Pselectin in serum and pulmonary tissue and alleviate the pathologic damage of the pancreas and lungs in rats with SAP complicated with lung injury, which suggests that Qingyi Ⅱ may play an important role in treating SAP. 　　【Keywords】 severe acute pancreatitis；lung/injury；rats；P selectin