温固化水凝胶的制备和应用_医学论文.docVIP

温固化水凝胶的制备和应用_医学论文 温固化水凝胶的制备和应用_医学论文 作者：李石保　杨维东　唐立辉　赵信义　陈萍 【关键词】 水凝胶 　　关键词: 水凝胶软骨组织工程 　　摘 要:目的 制备出适合于组织工程用的温固化型水凝胶，并将其应用于可注射软骨的建造，观察实验结果. 方法 制备不同浓度的水凝胶，并添加不同的物质，测量其凝胶化温度，并把水凝胶用于裸鼠组织工程软骨的建造.注射后4，8和12wk取材，通过大体标本和组织学观察研究软骨的形成情况. 结果 250g#12539L-1 的水凝胶具有恰当的凝胶化温度和能满足要求的力学性能.含钙磷的添加物因溶解度不高，对凝胶的性能未能产生显著影响.此水凝胶用于软骨组织工程，注射后4wk在皮下形成稍硬的结节.8wk后形成的结节质地坚韧，切面色泽亮白.组织学观察4wk即有软骨形成，但不成熟，8wk标本有成熟软骨形成，12wk标本成熟软骨的量进一步增加. 结论 温固化型水凝胶凝胶化温度受浓度影响，250g#12539L-1 水凝胶凝胶化温度为5℃，可用于组织工程软骨的建造，并能顺利长出软骨. 　　Keywords:hydrogelcartilagetissue engineering 　　Abstract:AIM To prepare temperature dependent hydrogel and to apply it as the scaffold in fabrication of tissue engi-neering cartilage and to observe the results.METHODS　Various concentration and additives of hydrogel were pre-pared，the gelation temperature were determined，the most favorite hydrogel（250g#12539L-1 ）was selected to be used as the scaffold of tissue engineering，and then the cartilage was fab-ricated.The chondrongenesis was studied histologically for12weeks.RESULTS Gelation temperature of250g#12539L-1 hydrogel was5℃，and its mechanical properties were good.However the solubility of additives was too low.The250g#12539L-1 hydrogel was applied in tissue engineering4weeks after injection，the hard nubble was formed subcutaneously and immatural cartilage was observed.8weeks after injection matural catilage was observed.12weeks after injection the amount of matural cartilage increased.CONCLUSION Gelation temperature depends on the concentration of hyd-gogel，the gelation temperature of250g#12539L-1 hydrogel being5℃.The hydrogel could be applied in fabrication of tissue engineering cartilage，and the cartilage could be fabricated successfully. 　　0 引言 　　聚氧化乙烯-聚氧化丙烯-聚氧化乙烯（PEO-PPO-PEO）三嵌段共聚物具有优异的生物相容性，在冰淇淋和乳状液的制造中用作增稠剂、凝胶剂、乳化剂、成型剂.它的另一个用途是在药物和化妆品制备中用作有一定活性的物质和油性物的溶剂.它具有优异的流体性质，稀的PEO-PPO-PEO水溶液为牛顿流体，当浓度大于100g#12539L-1 时，流动性明显变差，转变为塑性流体.大于100g#12539L-1 的水溶液都有一个凝胶化温度，当温度低于凝胶化温度时，粘度很低，为粘性流体，温度超过凝胶化温度时，粘度急剧增高，变为凝胶.Cabana等［1］ 详细研究了共聚物水溶液的凝胶过程，他们利用线性粘