清肺化痰汤合红霉素对 COPD 痰热阻肺证大鼠模型的疗效及机理探讨_医学论文.docVIP

清肺化痰汤合红霉素对 COPD 痰热阻肺证大鼠模型的疗效及机理探讨_医学论文 清肺化痰汤合红霉素对 COPD 痰热阻肺证大鼠模型的疗效及机理探讨_医学论文 作者：黄美杏 龚享文 陈斯宁 姚玉兰 张华玲 【摘要】 目的：探讨清肺化痰汤合红霉素对慢性阻塞性肺疾病（COPD）大鼠血清、肺泡灌洗液（BALF）中白介素-8（IL-8）表达及气道炎症的影响。方法：将 40 只 SPF 级 Wistar 大鼠随机分为 4 组，采用复合因素造模法建立COPD 大鼠模型，以观察清肺化痰汤合红霉素对 COPD 大鼠血清、BALF 中 IL-8 表达及气道炎症的影响。结果：与空白组比较，模型组各大鼠小气道出现组织细胞损伤、管壁炎症细胞浸润、血管增生及肺泡的融合，血清及 BALF 的 IL-8 的含量明显上升；与模型组比较，红霉素组在应用红霉素干预后大鼠小气道出现组织细胞损伤、管壁炎症细胞浸润、血管增生及肺泡的融合有所好转，血清及 BALF 的 IL-8 的含量明显下降，具有统计学意义；红霉素+清肺化痰汤组较红霉素组在同样的检测指标有明显下降。结论：清肺化痰汤合红霉素可以降低血及 BALF 中 IL-8 的表达，明显的抑制气道炎症的改变，提示清肺化痰汤具有治疗 COPD 气道炎症的作用。 【关键词】 清肺化痰汤；COPD；IL-8；气道炎症 〔Abstract〕 Objective To discuss the effects of Qingfei Huatan decoction combined with erythromycinin COPD (chronic obstructive pulmonary disease) model rats, including IL-8 in serum、BALF and airway inflammation. Methods 40 SPF level Wistar rats were randomly divided into 4 groups, COPD model rats were induced by complex factors, which is in order to observe the effects of Qingfei Huatan decoction combined with erythromycin on the expression of IL-8 in serum、BALF and airway inflammation. Results Compared with control group, model group appeared cell injury in small airway, inflammatory wall cells infiltration, angiogenesis and integration of alveoli, IL-8 levels in serum and BALF increased significantly compared with those of model group, after the intervention, small airway tissue injury, wall infiltration of inflammatory cells, angiogenesis and integration of alveoli were all improved, IL-8 levels in serum and BALF decreased significantly in erythromycin group compared with those of erythromycin group, indices of erythromycin + Qingfei Huatan decoction group were decreased significantly. Conclusion Qingfei Huatan decoction combined erythromycin can reduce the expression of IL-8 in serum and BALF, significantly inhibit the change of airway inflammation, suggesting Qingfei Huatan decoction can treat COPD airway inflammation. 〔Key Words〕 Qingfei Huatan decoction COPD IL-18 Airway inflammation 1 材料与方法 1.1 实