男宝片的健脾作用研究_药学论文.docVIP

男宝片的健脾作用研究_药学论文 男宝片的健脾作用研究_药学论文 【摘要】 　　目的研究男宝片的补肾健脾作用。方法实验观察男宝片对小鼠和大鼠的抗应激反应以及免疫器官重量的影响，探讨男宝片的健脾作用。结果男宝片能抑制小鼠小肠推进性运动，即由（88.4±4.3）%降为（71.1±9.0）%～（75.5±5.0）%。以ig大黄(1.0 g/只)qd×8 d造成脾虚模型(下同),以使脾虚小鼠耐常压缺氧时间、低温游泳时间、耐高温(45℃)时间、耐寒时间（-5℃）明显延长；使脾虚小鼠的脾脏和胸腺等免疫器官重量增加（中剂量优佳）；治疗4～8 d后使小鼠体温和体重高于模型组。以1.25 g/100 g大黄煎液给大鼠ig.qd×8d造成脾虚模型,可使脾虚大鼠尿木糖排泄率和血清木糖高于单纯脾虚模型组。结论男宝片有健脾功效。 【关键词】 男宝片 健脾 生大黄 小鼠 　　Abstract:ObjectiveTo study the effect of Nan Bao tablet of strengthening spleen and tonifying kidney.MethodsTo discuss the spleen strengthening effect of Nan Bao tablet by observing its impact on anti-stress reaction and weight of immune organs of rats and mice. Results(1)This preparation could inhibit the propelling movement of mices small intestine 〔Dropped from 88.4 ± 4.3%) to (71.1± 9.0)%～(75.5±5.0)%〕. (2)Using spleen deficiency model created by 1g rhubarb (1g/rat) qd × 8d, it showed that this preparation significantly lengthen the anti anoxia time under normal pressure, swimming time under hypothermia, high temperature tolerance time (45℃) and low temperature tolerance time (-5 ℃). It also increased the weight of immune organs such as spleen and thymus of spleen deficient mice (Especially using middle dose). 4～8 days after treatment, the body temperature and weight of those mice were all higher than those of model group. With spleen deficiency mode created by 1.25g/100g rhubarb decoction in rat qd×8d, this preparation could make urinary xylose excretion rate and serum xylose of spleen deficient rat higher than those of pure spleen deficient model group. ConclusionNan Bao tablet indeed has spleen strengthening effect. 　　Key words:Man Bao tablet; Strengthen spleen; Rhubarb; Mice 　　男宝片是根据民间验方，选用三十多种珍贵药材精制而成的名贵中成药。具有强身健脑、固肾补气的功效，主要用于腰膝酸软，头晕耳鸣，神疲乏力等症。近年来，我国加工生产的中成药男宝已畅销日本市场，日本田边制药也生产男宝供应临床。本实验探讨男宝片的健脾作用及机制。报道如下。 　　1 材料与仪器 　　1.1 药品与试剂男宝片，石河子市中医医院研制，新疆维吾尔药厂生产，每片相当于含生药0.41 g,批号为990421，使用时将药片研磨，加双蒸水配成每毫升含药0.025，0.05，0.1 g或0.035，0.07，0.14 g的混悬液，古汉养生精片，湖南古汉集团股份有限公司产品，每片0.4 g,(97)卫药准字Z-125号,具补肾益脾、健脑安神