番茄红素对急性肺损伤大鼠肺泡巨噬细胞影响_医学论文.docVIP

番茄红素对急性肺损伤大鼠肺泡巨噬细胞影响_医学论文 番茄红素对急性肺损伤大鼠肺泡巨噬细胞影响_医学论文 【关键词】 番茄红素；急性肺损伤；肺泡巨噬细胞；吞噬功能 　　摘要： 目的 观察番茄红素对急性肺损伤大鼠肺泡巨噬细胞吞噬功能的影响。方法 以05，10，30mg/(kg#12539bw)的番茄红素连续给雄性Wistar大鼠灌胃30d，用脂多糖制造大鼠急性肺损伤模型，分别在1，4，6h后支气管肺泡灌洗，检测肺泡巨噬细胞吞噬功能、细胞计数以及肺相对重等指标。结果 在各剂量番茄红素作用下，肺泡巨噬细胞吞噬功能明显增强，与模型组相比，注射脂多糖1h后，喂饲番茄红素组吞噬功能增强，增强率分别为112%，279%，381%4h后，增强率分别为59%，44%，170%；6h后，增强率分别为166%，152%和219%。番茄红素3个剂量组之间呈剂量反应关系(005)。结论 番茄红素在05，10，30mg/(kg#12539bw)剂量下能增强肺泡巨噬细胞的吞噬功能。 　　关键词： 番茄红素；急性肺损伤；肺泡巨噬细胞；吞噬功能 　　Effect of lycopene on function of pulmonary alveolar macrophages of rats acute lung injury 　　Abstract： Objective To study the effect of lycopene on the function of pulmonary alveolar macrophages(PAMs)of rats acute lung injury(ALI).Methods Male rats were gavaged continuously by lycopene with 05,10,30mg/kg for 30 daysLipopolysaccharide was used to make the model of ALI rats.After 1hour,4 and 6hours,bronchoalveolar lavage was done，and the function of PAMs,the amount of PAMs and the lung relative weight were determined.Results Lycopene could increase the phagocytic function of PAMs significantly .Compared with the model group,the phagocytic function of PAMs was increased significantly in lycopene groups,with increasing rates of 112%,279%,381% after injection of LPS for 1 hour,with increasing rates of 59%,44%,170% after injection of LPS for 4 hours,and with increasing rates of 166%,152%,219% after injection of LPS for 6 hours,respectivelely compared with the model group.The phagocytic function of PAMs was increased obviously by three different dose of lycopene,showing a doseresponse relationship(005).Conclusion Lycopene,at the dose of 05,10 and 30mg/kg,could reinforce the phagocytic function of PAMs. 　　Key words： lycopeneacute lung injury(ALI)pulmonary alveolar macrophages(PAMs)；phagocytic function 　　研究显示，番茄红素具有抗氧化、预防肿瘤和心血管疾病以及调节机体免疫功能等多种生理功能，是良好的抗氧化剂〔1-6〕。为观察番茄红素对脂多糖(lipopolysaccharide,LPS)致急性肺损伤(acute lung injury,ALI)大鼠肺泡巨噬细胞(pulmonary alveolar macrophages,PAMs)吞噬功能的影响，为番茄红素的开发提供基础数据进行了本研究。 　　1 材料与方法 　　11 主要仪器与