黄连解毒汤半仿生法提取工艺条件的优选_黄延亮.pdfVIP

食品与药品 Food and Drug 2011年第 13卷第05期 175 黄连解毒汤半仿生法提取工艺条件的优选 * 黄延亮，陈丽静，孙秀梅 ，张兆旺，王京龙，荆汉卫 （山东中医药大学，山东 济南 250014 ） 摘 要：目的 优选黄连解毒汤方药的提取工艺条件。方法 采用半仿生法，在药材规格、煎煮温度、煎煮用水量等条件 相同的情况下，用均匀设计U 1 3 9 （9 ×3 ）表，以盐酸小檗碱、黄芩素、栀子苷、总生物碱、总黄酮、HPLC总面积、相对 ≤ 分子质量 1 000的提取物为指标，综合评价，优选该方药的提取工艺条件。结果 三煎用水的pH依次为A=2.061 4，B=7.051 7， C=8.964 2 ；提取总时间D=4.968 0 。结论 结合生产实际，确定该方药用半仿生法提取的工艺条件为：3煎用水的pH值依次 为2.0，7.0，9.0，提取时间依次为2.0，1.5，1.5 h 。 关键词：黄连解毒汤；半仿生提取法；均匀设计；多成分指标；综合评价 中图分类号：R284.2 文献标识码：A 文章编号：1672-979X （2011）05-0175-06 Optimization of Technical Conditions for Huanglian Jiedu Decoction by Semi-bionic Extraction HUANG Yan-liang, CHEN Li-jing, SUN Xiu-mei, ZHANG Zhao-wang, WANG Jing-long, JING Han-wei (Shandong University of TCM, Jinan 250014, China) Abstract: Objective To optimize technical conditions for Huanglian Jiedu decoction by semi-bionic extraction. Methods The optimum extraction conditions were comprehensively evaluated and screened by uniform design U9 1 3 (9 ×3 ), maintaining the same traditional Chinese medicine specification, extraction temperature and extraction water volume. The contents of berberine, baicalin, geniposide, total alkaloid, total flavonoid, total integral calculus area of HPLC and extract with MW ≤ 1000 Da were used as evaluating indicators. Results The optimum pH values of 3 times of decoction water were A = 2.0614, B = 7.0517 and C = 8.9642; the total extraction time was D = 4.9680. Conclusion Combined with the production practice, the optimum extraction conditions for Huanglian Jiedu decoction were as follows: pH values of 3 times of decoction water were 2.0, 7.0 and 9.0; the extraction time was 2.0 h, 1.5 h and 1.5 h. Key Words: Huanglian Jiedu decoction