啤酒用β-葡聚糖酶高产菌株的选育及发酵条件优化.pdfVIP

农业生物技术学报 Journal of Agricultural Biotechnology 2004,12 (3): 316~321 ·研究论文· 啤酒用 - 葡聚糖酶高产菌株的选育及发酵条件优化* 元平 袁康培** 冯明光 (浙江大学微生物研究所，杭州310029) ： 摘要 对产 - 葡聚糖酶的地衣芽孢杆菌( ) A302 菌株进行紫外线和硫酸二乙酯(DES)复合诱变和选 育，获得产 - 葡聚糖酶达27.3 U/mL 的突变株 采用均匀 验设计对 菌株产 - 葡聚糖酶的液体培养 组成及发 酵条件进行优化实验，所获优化配方(W/V)为麦麸 1.4 1%，鱼粉0.80% ，硝酸钾0.34% ，硫酸镁0.11%，起始pH 6.0 ；用含孢量 108 个/mL 的种子菌液按体积比3.3%接种上述液体培养基，在36 下发酵52 h ，菌株 的产酶活力达到 115.1 U/mL ，是原出 发菌株及培养条件下产酶活力的9.4 倍。对突变株 所产 - 葡聚糖酶性质的研究表明，该酶最适作用温度为60 ，最适 作用pH 为5.5 ，适用于啤酒生产中的麦芽糖化。 ： ；均匀设计；发酵 关键词 - 葡聚糖酶；菌种选育；物理化学诱变 Breeding of a Bacterial Strain for Efficient Production of Beer-purpose -glucanase and the Optimized Conditions for Its Fermentation ZHENG Yuan-Ping YUAN Kang-Pei** FENG Ming-Guang (Institute of Microbiology, Zheji ang University, Hangzhou 3 10029, China) A mutant was generated from mutagenesis of strain A302 by inducing ultraviolet radiation and treating with 1% diethyl sulfate solution and could produce -glucanase at the level of 27.3 U/mL. Based on uniform experimental design and data analysis, components of an ideal liquid medium (W/V) for maximal production of -glucanase included 1.4 1% wheat bran, 0.80% fish meal, 0.34% potassium nitrate and 0.11% magnesium sulfate. The optimal conditions for 8 fermentation were