lgaris生物量和总脂肪酸含量的影响的研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2009, Vol.25, No.5 Effect of Metal Ions on Biomass and Total Fatty Acids Content of Chlorella vulgaris HUANG Guan-hua, CHEN Feng （College of Light Industry and Food sciences, South China University of Technology, Guangzhou 510640, China ） Abstract: Eight metal ions were studied for their contributions on biomass and the total fatty acid content (TFAC) of C. vulgaris cultivated heterotrophically in the dark. Cu2+, Zn2+ were limited nutritional factors for the alga growth and the biomass can be improved from 1.24 g/l to 3.60 g/l by adding 0.08 mg/l CuSO ·5H O. Similarly, the biomass can be improved from 1.71 to 3.96 g/l by adding 0.2 mg/l 4 2 ZnSO ·7H O. Under the conditions without adding Mg2+, the highest TFAC can be obtained, but the biomass was decreased. Mo6+, Mn2+ and 4 2 3+ 2+ Fe showed little effect on biomass and the TFAC, indicating that they had no special contribution to the growth of C. vulgaris. Increasing Fe concentration enhanced the TFAC of C. vulgaris. As for Ca2+, the maximal biomass and TFAC can be obtained in the medium containing 50 mg/l and 100 mg/l CaCl ·2H O respectively. 2 2 Key words: Chlorella vulgaris , Metal ion, Biomass, Total Fatty Acid Content (TFAC) CLC number: Q949; Document code: 1673-9078(2009)05-0474-05 金属离子对小球藻Chlorella vulgaris 生物量和总 脂肪酸含量的影响的研究 黄冠华，陈峰