GnRHa对子宫内膜异位症内膜间质细胞生长增殖及血管形成的影响_韩利伟.pdfVIP

2008 10 1 10 Prog Obstet Gynecol, Oct. 2008, Vol11 , No1 10 # # GnRHa 韩利伟, 姜卫国 (山东大学附属千佛山医院, 济南 250014) = : 研究GnRH a对子宫内膜异位症异位及在位内膜间 细胞增殖凋亡 及血管形成的影响: 体外原代培养人内异症异位及在位子宫内膜间 细胞, 用不同 浓度的 GnRHa分别干预, 四甲基偶氮噻唑蓝 (MTT )法测定内膜间 细胞的生长增殖活 力; Annexin V-FITC和 PI双染流式细胞仪( FCM )检测异位及在位内膜间 细胞凋亡及 周期变化; 免疫细胞化学检测血管内皮生长因子(VEGF)表达的改变: GnRHa可抑 制内异症异位及在位内膜间 细胞增殖且呈剂量依赖关系; GnRH a能明显增加异位及在 位内膜细胞的凋亡率(P 0. 05), 且异位内膜细胞凋亡率明显高于在位内膜(P 0. 05); 不同浓度的 GnRHa作用后, 内膜间 细胞均发生细胞周期阻滞, G 期细胞增加, S期细胞 1 减少, 此作用随浓度增加而增强(P 0. 05); 免疫细胞化学测定表明, GnRH a可降低异位 及在位内膜间 细胞 VEGF的表达(P 0. 05), 且随GnRH a浓度增加而增强(P 0. 05) : GnRHa可明显抑制人内异症异位及在位子宫内膜间 细胞的生长并促进其凋亡, 降低异位及在位内膜间 细胞 VEGF的表达 = 促性腺素释放激素; 在位内膜间 细胞; 异位内膜间 细胞;血管内皮生长因子 : R 3 . 33 : A : 1004- 3 9( 2008) 10- 0 3 - 04 Effect of GnRHa on the proliferation and angiogenesis of ectopic and eutopic stromal cells.H an L iwei, J iang Weiguo. The aff iliated Qianf oshanH osp ital of Shandong Un iversity, Jinan 250014 =Abstract Objective: To investigate the effect of GnRHa on the proliferation and an- giogenesis of ectopic and eutop ic endometrial stromal cells from w omen w ith endometriosis in vitro. M ethods: The ectop ic and eutop ic endometrial stromal cellsw ere isolated and cultured in vitro, then exposed to d ifferent concentration ofGnRH a. The grow th and proliferation of ectop ic and eutop ic strom al cellsw eremeasured byMTT assay. The cell cycle d istribution and apoptosis of ectop ic and eutop ic endom etrial stromal cellsw eremeasured by flow cytometryw ith Annexin V-FITC and PI double stain ing. The expression of VEGF induced by GnRHa w as analyzed by mi munocytochem istry. Results: GnRH a could inh ib it the proliferation of ectopic and eutop ic en- dometrial stromal cells from w omenw ith e