pRNA介导的RNA干扰抑制HBV表达和复制的研究.pdfVIP

第 21 卷 5 期 中 国 病 毒 学 21 （6 ）：599-603 2006 年 11 月 VIROLOGICA SINICA November 2006 pRNA 介导的 RNA 干扰抑制 HBV 表达和复制的研究* 1 1 1 1 2 1** 张正茂 ，田拥军 ，杨 燕 ，王宝菊 ，郭培宣 ，杨东亮 (1.华中科技大学同济医学院附属同济医院临床免疫研究室，湖北武汉，430030 ；2. Department of Pathobiology and Purdue Cancer Research Center, Purdue University, West Lafayette, IN 47907, USA) Inhibition of Hepatitis B Virus Replication by pRNA-escorted siRNA 1 1** ZHANG Zheng-mao , TIAN Yong-jun, YANG Yan, WANG Bao-ju, GUO Pei-xuan, YANG Dong-liang (1.Division of Clinical Immunology, Tongji Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China; 2. Department of Pathobiology and Purdue Cancer Research Center, Purdue University, West Lafayette, IN 47907, USA)) Abstract ：The objective of our present study is to explore the potential use of pRNA as a bio-carrier of siRNA to inhibit HBV gene expression and replication. After co-transfected with pHA-HBs into 293T cells, HBVsi18-42, a pRNA-escorted siRNA, suppressed HBsAg and accumulated in the cells in a dose-dependent fashion. HBVsi18-42 substantially inhibited HBV gene expression and replication initiated by pHBV1.3 in HepG2 cells. In hydrodynamic injection mouse model, Balb/cJ mice were co-injected with pHBV1.3 and HBVsi18-42. Serum concentrations of HBsAg were analyzed by ELISA on days 1, 2, and 3 post-injection. HBV core protein in mouse liver was visualized by immunohistochemical staining. The results showed that the