Survivin拮抗肽的筛选及鉴定.pdfVIP

2007 7 7 14 , 2007 7 . 14 3188 Chin J M is diag n Jul V ol N o 【研究原著】 Surv ivin 1 1 2 1 1 窦长武, 田复明, 王晓娟 , 高乃康 , 武文元 : Sur vivin , , Sur vivin : 12 Surv iv in , ; W w est ern blot ca spase-3 Surv iv in : Surv iv in , 8 ; , 8 Sur viv in , 3 ( P 0. 05) ; 3 caspase -3 : Sur vivin , / ; / ; R979 . 1A 1009-6647( 2007) 14 -3 188-0 3 Bio-panning and Identif ication of Antagonistic Peptides Against Survivin DOU Chang -w u , T I AN F u -ming , W AN G Xiao - ua n, et al . ( , , 010050 ) Dep artment of N eur osurg ery The First Af f iliat ed Hosp ital of I nner Mongolia Med ical Colleg e Hohhot China A bstractObj ective : T o scr een out the Surv iv in binding peptides and f ind o ut an antago nist ag ainst it . Methods : T he Surv ivin binding peptides w er e acquir ed by bio -pa nning a phag e-display ed 12-peptide librar y . Aft er obtaining t he se- quence o f the selected phag e-display ed peptides, all the 8 peptides w ere synthesized by so lid-phase method and identif ied . - by mass spect ro gr aph T he peptides anti Sur vivin expr essio n and functio n w er e detected by W est er n blot a nd testing caspase-3 activ ity . Results : Aft er 3 r ounds of bio -panning the phag e-display ed pept ide libr ary , 8 po sitiv e phag e clones w ere select ed and sequenced . T he synthesized peptides had uneven inh