红曲霉菌产MonacolinK固体发酵条件的优化_熊晓辉.pdfVIP

第23 卷第1 期 无锡 轻 工大学 学 报 Vol. 23 No. 1 2004 年 1月 Journal of Wuxi University of Light Industry Jan. 2004 : 1009-038X(2004) 01-0009-05 红曲霉菌产Monacolin K 固体发酵条件的优化 1 2 1 1 熊晓辉 , 张李阳, 韦策 , 熊强 ( 1. 南京工业大学 制药与生命科学学院, 江苏 南京210009; 2. 南京晓庄学院, 江苏南京210006) : 对M onacus sp p . 3 的固体发酵生产Monacolin K 的培养条件和培养基进 了优化. 考察 了物料厚度起始含水量发酵时间起始pH 值等发酵条件,以及大米培养基中添加附加碳源氮 源磷酸盐等的影响. 结果表明, 固体发酵的培养条件为: 物料(大米)厚度在4 c 以下,起始pH 值 自然,培养温度32 , 相对湿度60%~ 70%, 发酵时间为12 d; 经单因素和正交试验得适宜的发酵 培养基为:大米 100 g, 甘油0.2 g, 玉米浆0. 15 g,起始含水量50%. 且以此优化条件固体发酵培 养, Monacolin K 产量稳定在 1.4 g/ g 左右, 最高产量为1.6 g/g. : 红曲霉; Monacolin K; 固体发酵; 优化 :T Q 925 : A Optimizing Solid Fermentation of Monascus spp . 3 for Producing Moncolin 1 2 1 1 XIONG Xiao-hui , ZHANG Li-yang , WEI Ce , XIONG Qiang ( 1. College of Life Science and Phar aceutical Engineering of Nanjing University of Technology, Nangjing 210009, Chi- na; 2. Life Science Depart ent of Nanjing Xiaozhuang Institute, Nangjing 210006, China) Abstract: The ediu co ponents and fer entation conditions for the Monacolin K production by M onacus spp . 3 solid fer entation were studied. The operating factors, including the thickness of rice, initial water content, fer entation ti e, initial pH, appendant carbon and nitrogen source, and the concentration of K HPO were investigated. The opti al fer entation conditions were deter ined 2 4 as follow: the thickness of aterials 4.0 c ; natural initial pH; culture te perature, 32 ; relative hu idity, 60~ 70%; and fer entation ti e, 12 days. The fe