运用分子生物学技术监测水环境中产毒蓝藻.pdfVIP

Chinese Journ l of Ecology 2011, 30( 2) : 363- 368 * 朱 鹏 严小军* * ( 宁波大学应用海洋生物技术教育部重点实验室, 浙江宁波 3152 11) 有毒蓝藻在形成水华破坏水体环境的同时, 也对人畜产生危害运 分子生物 学技术对水环境中产毒蓝藻进行监测, 由于其简单快速和经济等优点, 逐渐成为各国学者 关注的热点本文从以下 3 个方面对其进行了综述: 1) 早期分子生物学技术在产毒蓝藻诊 断中的运 ; 2)以产毒基因为靶标进行的产毒蓝藻诊断; 3) 运 基因芯片技术对产毒蓝藻 进行检测与传统的分子生物学技术相比, 生物芯片技术具有体积小重量轻便于携带 分析自动快速高通量等许多传统方法所不能比拟的优势因此, 该技术在蓝藻毒素检测 中的运 必将给目前的产毒蓝藻的检测带来一场新的革命 产毒蓝藻; 产毒基因; PCR; 基因芯片; 监测 Q9388 A 1000- 4890( 2011) 2- 0363- 06 Applications ofm olecular biotechnology in m onitoring toxic cyanobacteria in water envi * * ronm ent. ZHU Peng, YAN X i ojun (K ey L a boratory of App lied M arine B iotech nology, N ing bo Un iversity, N ing bo 315211, Zhej iang, Ch ina ) Ch inese Journal of E cology, 2011, 30 ( 2) : 363- 368. Abstract: Tox ic cy nob cteri c n form b loom s in fresh, br ck ish, nd m rine w ters, giving thre ts to w ter environment, nd lso, to hum n nd n mi l he lth. Ow ing to its smi p le, f st, nd econom ic dv nt ges, molecul r biotechnology h s become the focus of concern in mon itoring tox ic cy nob cteri in w ter environm ent. Th is p per summ rized the rese rch progress in th is field from the spects of 1) mon itoring tox ic cy nob cteri by using tr dition lmolecul r b iotech nology, 2) molecu l r detection ccord ing to the synthet se gene of cy notox in, nd 3) detection of tox ic cy nb cteri by using DNA m icro rr y technology. Comp redw ith conven tion lmolecu l r biotechnology, the DNA m icro rr y technology offers r pid, econom ic, nd h ighthroughout op tion for detecting tox ic genotypes in co