SRAP在检测黄瓜基因组多态性中的特征.pdfVIP

遗 传 HEREDITAS Beijing 28 11 : 1435~1439, 2006 研究报告 DOI: 10.1360/yc-006-1435 SRAP 在检测黄瓜基因组多态性中的特征 1 2 1 2 1 2 1 钱文成 , 张桂华, 陈飞雪, 韩毅科, 陈德富, 杜胜利 , 陈喜文 1. 南开大学生命科学学院, 天津 300071; 2. 天津科润农业科技股份有限公司黄瓜研究所, 天津 300192 摘 要: 将 SRAP Sequence-Related Amplified Polymorphism 应用于黄瓜 Cucumis sativus L. 遗传图谱和耐高温 QTL 的定位过程中, 发现 SRAP 在检测黄瓜亲本基因组多态性中呈现出一些特征。对于每个正向引物, 在与 12 个不 同的反向引物组合时, 产生多态性条带的引物组合数均在 5~8 个之间; 而对于每个反向引物, 在与 11 个不同的正向 引物组合时, 产生多态性的引物组合数则在 2~11 个之间, 差异较大。反向引物 SA4 或 EM6 与研究的所有正向引物 组合时产生的多态性条带分子量完全相同, 这些条带可能是由反向单引物扩增而来的。引物组合 OD3ME11 扩增出 的多态性条带存在共分离现象。同时对利用 SRAP 的这些特征指导我们的研究进行了讨论。 关键词: 黄瓜; SRAP; 分子标记; 特征 中图分类号: Q78 文献标识码: A 文章编号: 0253-9772 2006 11-1435-05 Characteristics of SRAP Marker in Detecting Polymorphism of Cu- cumber Genome 1 2 1 2 1 QIAN Wen-Cheng , ZHANG Gui-Hua , CHEN Fei-Xue , HAN Yi-Ke , CHEN De-Fu , DU Sheng-Li2 , CHEN Xi-Wen1 1. College of Life Sciences , Nankai University , Tianjin 300071, China ; 2. Cucumber Research Institute , Tianjin Kerun Agriculture Co . Ltd , Tianjin 300192, China Abstract: When SRAP Sequence-Related Amplified Polymorphism marker was used in constructing genetic map and analyzing QTL for high temperature resistance in cucumber Cucumis sativus L. , it exhibited certain characteristics in detecting genomic polymorphism. When each forward primer was combined with different reverse primers, the number of primer combinations that produced polymorphism ranged from five to eight. When each reverse primer was in combination with different forward primers, the number of polymorphic primer combinations ranged from two to eleven. The reverse primers SA4 or EM6 produced identical polymorphic bands when combined with all the forward primers tested. These bands might be amplified by the reverse primers. The polymorphic bands amplified from OD3ME11 co-segregated in the F2 population. The utilization of these characteristics in our research was discussed. Key wo