-Presented by-Peter Oledzki Joh.pptVIP

Aims of Proteomics Detect the different proteins expressed by tissue, cell culture, or organism using 2-Dimensional Gel Electrophoresis Store those information in a database Compare expression profiles between a healthy cell vs. a diseased cell The data comparison can then be used for testing and rational drug design. Gel Electrophoresis Motion of charged molecules in an electric field. Polyacrylamide gel provides a porous matrix (PAGE – Polyacrylamide Gel Electrophoresis) Sample is stained with comassie blue to make it visible in the gel. Sample placed in wells on the gel. 1-D Gel electrop