愈肾合剂对糖尿病肾病大鼠肾脏细胞凋亡及ERK信号转导系统的影响.pdfVIP

ERK 4 607 ERK 常 庚 成秀梅 王晓松 路 帅 (河北医科大学中西医结合学院, 河北 石家庄 050091) ( DN) ERK SD ( STZ) DN, , 8 w caspase9 caspase3, W estern ERK 1/2 DN, caspase9 caspase3 , ERK ; ; ; ; caspase9; caspase3; ERK R2855 A 2011 Effect of Shenyuheji on kidney cell a o tosis and ERK signal transduction system of diabetic ne hro athy rat CHANG G eng, CHENG X iuM ei, WANG X iaoSong, e t a l Second Hos ital ofHebeiM edicalUniversity, College of Integrated Traditional andW esternM edicine ofH ebeiM edicalUniversity, Shijiazhuang 050091, Hebe,i China Abstract Objective To o serve the effect of Shenyuheji on kidney cell apoptosis and ERK signal transduction system of d ia etic nephropathy ( DN ) ratM ethods DN SD m odel ratsw erem ade y streptozotocin ( STZ) lum ar injection and unilateral nephrectomy and w ere feedw ith Shenyuheji gastric perfusionC ell apoptosis rate and the expressions of caspase3 and 9 were detected y flow cytom etryThe expression of phosphorylation ERK 1/2 proteinw as m easured yW estern lottingResults Shenyuheji could decrease the num er of apop tosis cell in DN rats renal cortex, reduce the express ions of caspase3 and 9, and activate ERK signal pathw ay Conclusions Shenyuheji can efficiently reduce kidney cell apoptosis and activate ERK signal pathway Key words Prescription; Ch inesem aterial m ed icine; D ia etic nephropathy; Cell apoptosis; caspase9; caspase3; ERK , DN , 10% , ( DN), , 3 m l/kg, , , 15 cm, , , , , , 1~ 3 DN , , , 4 , 001 m l, 2 w caspase9 caspase3 ERK , , 50 m g/kg STZ( 01m ol/L, pH = 45 DN )48 h , , 167 mmol/L 1