Plackett-Burman设计和响应面法优化荷叶总黄酮的提取工艺.pdfVIP

※工艺技术 食品科学 2009, Vol. 30, No. 06 29 杨冀艳，胡 磊* ，许 杨 (南昌大学中德联合研究院，食品科学国家重点实验室，江西南昌 330047) 在单因素试验基础上，首先用Plackett-Burman 设计对影响荷叶总黄酮得率的因素进行了评价，筛选出具 有显著效应的三个因素——乙醇浓度、液固比和提取次数；然后用响应面分析法确定了主要影响因素的最佳提取条 件为乙醇浓度67% 、液固比34:1(V/m) 、提取3 次，在此条件下荷叶总黄酮得率的预测值可达3.19% ，实测值为 3.17% ，两者较接近。表明Plackett-Burman 设计结合响应面分析法可以很好地对荷叶总黄酮提取工艺进行优化。 荷叶；总黄酮；Plackett-Burman 设计；响应面法 Optimization of Extraction Conditions of Total Flavonoids from Lotus Leaves by Plackett-Burman Design and Response Surface Analysis YANG Ji-yan ，HU Lei* ，XU Yang (Sino-German Joint Research Institute, National Key Laboratory of Food Science, Nanchang University, Nanchang 330047, China) Abstract：On the basis of single-factor tests, some factors affecting the extraction rate of total flavonoids from lotus leaves were firstly evaluated by Plackett-Burman design, and then the extraction conditions were optimized by response surface analysis. The results showed that the optimal extraction conditions are as follows: ethanol concentration 67%, ratio of ethanol solution to raw material 34 ∶1 and extraction times 3. Under the above conditions, the predicted value of extraction rate of total flavonoids is 3.19% and its experimental value is 3.17%, which indicated that the combination of Plackett-Burman design and response surface analysis can well optimize the extraction technology of total flavonoids from lotus leaves. Key words ：lotus leaves ；total flavonoids ；Plackett-Burman design ；response surface analysis 中图分类号：R284.2 文献标识码：A 文章编号：1002-6630(2009)06-0029-05 荷叶(Folium Nelumbinis)是睡莲科植物莲(Nelumbo 率的主要因素，然后利用响应面分析法对主要因素进行 nucifera Gaertn) 的叶片，1991 年被列入第二批“既是 优化，确定较优的提取工艺条件，为大批量生产提供 食品又是药品