硫酸长春新碱缓释药膜的制备与性质研究.pdfVIP

硫酸长春新碱缓释药膜的制备及性质研究 * 陈红丽，陈汉，莫丽都尔，刘玲蓉，张其清 ( 中国医学科学院 中国协和医科大学 生物医学工程研究所, 天津市生物医 学材料重点实验室，天津 300192) 摘要 本研究制备载硫酸长春新碱微球的胶原－壳聚糖的缓释药膜。并考察加入壳聚糖对药膜性质的影响。选 定适当的胶原壳聚糖比例制备药膜。采用W/O/O溶剂挥发法制备硫酸长春新碱的PLGA微球，并对微球性质表征， 采用二次冻干法制备载硫酸长春新碱微球的胶原－壳聚糖药膜，对药膜的表面形态、降解性质，热力学性质、释放 性质进行表征，并与释放2周后的药膜进行比较。采用高效液相法分析药物含量。硫酸长春新碱制成PLGA微球后再 制备成药膜，可达到双重缓释的作用，明显减少药物突释，并延缓药物释放。添加了壳聚糖的药膜降解速度明显小 于单纯的胶原药膜。在体外释放实验中，微球突释为(27.2±1.2)%，而胶原药膜的突释为(20.4±1.9)％，胶原：壳聚 糖比例为9 ：1，4 ：1，3 ：2 的药膜突释分别为(20.2±2.1) ％、(18.0±1.1)％，(16.3±1.8)％。胶原壳聚糖载硫酸长春新 碱的缓释药膜能不同程度减少药物的突释，使药物释放更加平稳缓慢，优于单纯的胶原药膜。有望成为一种实用的 新型缓释抗肿瘤制剂。 关键词 硫酸长春新碱； PLGA; 微球； 胶原；壳聚糖，植入剂 The preparation and characteristics of collagen-chitosan complex film containing PLGA microspheres CHEN Hongli, CHEN Han, Moldir, LIU Lingrong, Zhang qiqing （Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin Key Laboratory of Biomaterials, Tianjin 300192, China ） Abstract Aim The objective of this study is to investigate the preparation and characterization of collagen-chitosan complex films. Methods A water-in-oil-in-oil double-emulsion /solvent evaporation method was used to prepare VCR-loaded PLGA microspheres, then microspheres were mixed with collagen and (or) chitosan swelling solution, and lyophilized.The films were cross-linked by 0.3% glutaraldehyde. Encapsulation efficiency and release kinetics of VCR microspheres were determined,as well as release kinetics and in vitro degradation of the film. The rate of VCR release from the film submerged in PBS (pH6.8) and the content were measured by high-performance liquid chromatography (HPLC). Results VCR was released from the film in a prolonged period and the initial burst release of the film was less significant.