基因差异表达分析.pptVIP

Other Methods- Comparison R-fold Method: Gene i is significant if r(i)R or r(i)1/R FDR 73%-84% - Unacceptable. Pairwise fold change: At least 12 out of 16 pairings satisfying the criteria. FDR 60%-71% - Unacceptable. Why doesn’t it work? Fold-change, SAM- Validation Summary SAM is a method for identifying genes on a microarray with statistically significant changes in expression. Developed in a context of an actual biological experiment. Assign a score to each gene, uses permutations to estimate the percentage of genes identified by chance. Comparison to other methods. Robust, can b