荧光标记来分析支链淀粉分子结构.pdfVIP

Carbohydrate Research 338 (2003) 471– 475 /locate/carres Note Examination of the structure of amylopectin molecules by fluorescent labeling Yasuhito Takeda,* Shunpei Shibahara,1 Isao Hanashiro Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima Uniersity, Kagoshima 890-0065, Japan Received 5 June 2002; received in revised form 21 October 2002; accepted 18 November 2002 Abstract Amylopectin molecules from rice, maize, sweet potato and potato were examined by fluorescent labeling followed by gel-permeation HPLC. The number-average degree of polymerization (dpn) was determined to be in range of 9600–15,900. The molar-based distribution revealed the presence of three molecular species, large (dpn 13,400–26,500), medium (4400–8400) and small (700–2100). Their molar proportions differed by plant origin. The large species was a major component (43–63% by mole). A relatively large amount of the medium (16–28% by mole) and small (19–38%) species was found although their weight proportion was small (8–15, 1–4%, respectively). The three species from waxy rice amylopectin had a similar chain-length distribution and also a similar size-distribution of C chains. These results suggested that the three species were basically similar in cluster structure but different in number of clusters per molecule. © 2003 Elsevier Science Ltd. All rights reserved. Keywords: Starch; Granule; Amylopectin; Fluorescent labeling; Gel-p