CT脑灌注剂量下X射线对体外培养的骨髓间质干细胞的影响.pdfVIP

14 卷 6 期 生命科学研究 灾燥造援员4 晕燥援6 圆园10 年 12 月 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Dec. 圆园10 CT 脑灌注剂量下X 射线对体外培养的骨髓间质 干细胞的影响 * 黄铀新 吕富荣 吕发金 马秀华 肖云华 肖智博 ， 重庆医科大学 附属第一医院放射科 中国重庆400016 ） 摘 要 观察大鼠CT 脑灌注剂量下的X 射线照射对体外培养的骨髓间质干细胞的影响. 体外大鼠培养骨髓 间质细胞 分为照射组和未照射对照组 照射组细胞分别进行 1~5 次大鼠脑灌注条件下CT 扫描. 两组细胞分 别做生长曲线 运用流式细胞仪检测细胞周期改变情 以及行噻唑蓝 渊3 渊4 5冤 dimethylthiahiazo 渊 z y1 冤 3 5 di phenytetrazoliumromide MTT 冤法检测细胞凋亡情 . 5 次大鼠脑灌注CT 扫描下照射组细胞活性及凋亡情 况与对照组细胞差异无统计学意义. 常规大鼠脑灌注CT 扫描对骨髓间质干细胞活性无影响. 关键词 骨髓间质干细胞曰 CT 脑灌注成像曰 细胞活性 中图分类号 R811.5 文献标识码 A 文章编号 1007 7847 渊20 10冤06 0534 04 Impacts of the Doses of X ray Radiation in CT Brain Perfusion on Rat Mesenchymal Stem Cells In vitro * HUANG You xin ，L譈 Fu rong ，L譈 Fa jin ，MA Xiu hua ，XIAO Yun hua ， XIAO Zhi bo Dep artment of Radiology ，First Affil iated Hosp ital ，Chongqing Medical Univ ers ity ，Chongq ing 400016 ，China） Abstract ：In order to investigate direct impacts of X ray radiation on cultured rat messenchymal stem cells under CT rat brain perfusion in v itro ，cultured rat marrow mesenchyma cells were divided into control group and irradiation group. Each irradiation groups were divided into 5 subgroups and underwent 1~5 scannings of CT brain perfusion respectively. After irradiation ，cellular growth curves were recorded ，and the cell cycle was detected by FCM ，as well as the cell viability was examined by MTT 3 4 ，5 ）dimethylthiahiazo z y1 ）3 ，5 di phenytetrazoliumromide ）assay. T