新型木聚糖酶在纸浆漂白中的应用.docVIP

新型木聚糖酶在纸浆漂白应用中的研究 乔婧1，樊帅1，符传学1 （工业发酵微生物教育部重点实验室 工业酶国家工程实验室 天津市工业微生物重点实验室 天津科技大学生物工程学院， 天津 300457） 摘要：本研究从天津广聚源造纸厂及其周边的碱性环境中采集样品，经分离筛选得到一株高产木聚糖酶的细菌，命名为Paenibacillus campinasensis G1-1(CGMCC No.5023)。将该基因连接于E.coli/B. megaterium穿梭载体pSTREPHIS1525上，并实现了其在Bacillus megaterium MS941中的分泌表达。纯化后的重组酶XynG1-1最适温度为60℃，最适pH为7，在80℃下保温3h残余酶活为32%，在pH10下保温3h残余酶活为58%。在酶用量为15 IU/g pulp，70℃、pH9条件下预处理3h，结果使后续的D0ED1化学漂白中的二氧化氯用量降低50%，并且其纸浆白度没有损失，纸浆的机械强度反而有所增强，可见，由基因工程菌B. megaterium MS941(pSH-XynG1-1)生产的重组木聚糖酶在纸浆漂白方面具有很好的应用前景。Bacillus megaterium MS941; 纸浆漂白； The Research on a New Xylanases Applied on Pulp Bleaching QIAO Jing1，FAN Shuai1，FU Chuan Xue1 （Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, National Engineering Laboratory for Industrial Enzymes, Tianjin Key Laboratory of Industrial Microbiology, College of Biotechnology,Tianjin University of Science and Technology, Tianjin 300457） Abstract: Sample were collected from Tianjin Paper Mill and Alkaline environment, and obtained a high yield of xylanase bacteria by isolation and screening, named Paenibacillus campinasensis G1-1(CGMCC No.5023). The xylanase gene (xynG1-1) from Paenibacillus campinasensis G1-1 was expressed in Bacillus megaterium MS941 and a high level of extracellular xylansae activity. Application of XynG1-1R (15 IU/g pulp) to cotton stalk pulp bleaching increased brightness by 3.65% over that of the control without the xylanase and reduced the need for chlorine compounds by 50% without loss of brightness and pulp fiber qualities the recombinant xylanase produced by genetically engineered bacteria B. megaterium MS941(pSH-XynG1-1) has a good prospect of application in pulp bleaching. Keywords: papermaking; xylanase; Bacillus megaterium MS941; pulp bleaching 引言 造纸工业是我国乃至其他一些国家最大的工业之一，同时也是国民经济的重要支柱产业之一，纸浆生产在给社会带来巨大经济效益和社会效益的同时，也极大地消耗了资源、能源，并造成了严重的环境污染。面对制约造纸工业生存的资源、能源和环境三大问题，只有坚持走节能减排、清洁生产的道路，才能促进造纸工业的可持续发展，国外造纸工业发达国家的前期成功实践已经证明了这一点。因此我国造纸工业也必须走中国特色的清洁生产道路，实现我国造纸工业的可持续生存和可持续发展