丙型肝炎病毒复合高区1模拟表位蛋白的免疫原性分析.pdfVIP

21 1 21 124-28 2006 1 VIROLOGICA SINICA January 2006 ** 200433 Immunogenicity of a Hepatitis C Virus Multiple-mimotope Protein * of Hypervariable Region 1 ** GONG Yu-ping, GAO Jun, ZHAO Ping, QIN Zhao-ling, YANG Miao, QI Zhong-tian Department of Microbiology, Key Laboratory for Medical Microbiology of PLA, Second Military Medical University, Shanghai 200433, China Abstract A synthesized multiple-mimotope gene of Hepatitis C virus was cloned into a prokaryotic expression vector pGEX-4T-1 to generate a fusion protein GST-HCVME. The reactivity of the GST- HCVME with the sera of HCV patients was measured by Western-blot and ELISA. BALB/c mice were injected with GST-HCVME, and an ELISA approach was applied to determine the specific antibody titers in the mouse serum. The cross-reactivity of the antibodies was also checked with 2 synthesized HCV hyper-variable region 1 (HVR1) peptides. The results showed that the purified GST-HCVME fusion protein was able to react with 25 out of 35 HCV patients’ serum samples. The serum antibody response was effectively elicited in BALB/c mice injected with GST-HCVME. The highest titer of 4 antibody against HCV (GST-HCVME) was about 1:10 at the eighth week after first immunization. Moreover, the collected mouse serum antibody had the ability to cross-react with the 2 synthesized HCV HVR1 peptides. These findings suggest