_互补与蓝白斑筛选机制的探究.pdfVIP

生物技术通报 BIO TE CHN OLOG Y B ULLETIN 20 10 8 周玉亭 曹建斌 (, 044000) : 针对高校生物科学实验教学中存在的问题, 着重探究了基因重组 验中的 互补与蓝白斑筛选机制, 精心设 计了 5组 验, 结果指出, 经双酶切的质粒载体自身不能环化, 也不能成功转化; 蓝白斑筛选中的蓝斑通常来源于商品载体中 的环状质粒或者线性载体自连后的转化产物; 重组子 一般为白斑, 但在 一定条件下, 白斑也会变成蓝斑在此基础上作了进 一步的讨论, 为今后的教学实践和科学研究提供借鉴和指导 : 互补 蓝白斑筛选 重组子 The Research intoM echanism of complem entation and B luewhite Screening Zhou Yuting Cao Jianbin ( Yuncheng Colleg e, D ep a rtm en t of Lfi e S cience, Yunch eng 044000) A bstrac:t Based on the questions of b iologi al expermi ents in ollege tea h ing, the resear h exp lored them e han ism of omp le m entation and bluewh ite s reen ing and pre isely designed 5 expermi ents. The resu lts show ed that p lasm id ve tor ould not form loop it self or transform su essfu lly. The b lue pat h an be ommonly from ir u lar plasm id or transform ation produ ts of selfligation. The re omb inants usually displayw hite pat hes, but the w hite pat hes an be om e blue under spe ifi ond itions. The resu lts provided d ire tions for future resear h and tea h ing pra ti e. K ey w ords: om plem entation B luewh ite s reen ing Re omb inan t ( gene engineering) 20 70 , , , DNA DNA , C , , DNA , Xgal DNA, , DNA , , , N , , , [ 1] E. coli ( N C) , , E. coli DNA ,