Bioreactor Analysis and OperationChapter 9amp;10 (textbook).ppt

Bioreactor Analysis and OperationChapter 910 (textbook) Overview of bioreactors Modified batch and continuous reactors Scale up/down Immobilized cell systems Operation consideration Sterilization Bioreactor Instrumentation and control Bioreactor Analysis and Operation Overview of bioreactors for submerged system - Classification: operation modes: - batch: stirred tank - continuous: chemostat, fluidized-bed - modified types of the above modes: fed-batch, chemostat with recycle, multi-stage continuous reactors Requirements for Cultivation Methods Biomass concentration which must remain high Sterile conditions being maintained Effective agitation so that the distribution of substances in the reaction is uniform Heat removal Creation of the correct shear conditions - high may damage cells, low may lead to flocculation or growth on wall and stirrer Chemostat with Cell Recycle - To keep the cell concentration higher than the normal steady-state level in a chemostat. - To increase the cell and growth-associated product yield. - For low-product-value processes: e.g. waste treatment. fuel ethanol Chemostat with Cell Recycle Chemostat with Cell Recycle Chemostat with Cell Recycle Chemostat with Cell Recycle Cell mass balance around the cell separator. Example-Chemostat with Cell Recycle Organisms are cultured in a chemostat with cell recycle. The system is operated under glucose limitation. Fed-Batch Nutrients are continuously or semi-continuously fed, while effluent is removed discontinuously. - overcome substrate inhibition or catabolite repression by intermittent feeding of substrate by maintaining low substrate concentration. for production of secondary metabolites e.g. antibiotics, lactic acid, E. Coli making proteins from recombinant DNA technology. Fed-Batch Fed-Batch Fed-Batch Fed-Batch Example: Fed- Batch In a fed-batch culture operating with intermittent addition of glucose, the value of V is given at time t=2hr, when