鸡胚绒毛尿囊膜数字图像血管定量分析.docVIP

鸡胚绒毛尿囊膜数字图像血管定量分析* 琚立华1，胡 平2，倪 爽3 Quantitative analysis of blood vessels in chick embryo chorioallantoic membranes through digital imaging Ju Li-hua1, Hu Ping2, Ni Shuang3 Abstract AIM: To study the method for automatic analysis of blood vessels in digital chick embryo chorioallantoic membrane (CAM) images and develop a special experimental analysis system based on digital image processing and biomedical engineering. METHODS: The experiment was performed at the Central Laboratory of School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, and Shanghai University of Traditional Chinese Medicine from June 2005 to June 2007. Based on the characteristics of CAM images, the image analysis software and hardware were studied and developed. The main functions of the analysis software included CAM image background fading, blood vessels enhancement, background and blood vessel calibration, measurement region division, micro-ruler calibration, blood vessel area and area ratio measurement and so on. The analysis method and measurement parameters were tested by experiments, and the analysis system was improved during that process. RESULTS: The analysis method and procedure as well as the analysis system for CAM image were efficiently established. The parameter measurements in CAM images from drug experiments in which the animals were divided into several groups were executed. The data obtained from the experiments were statistically analyzed. The result of statistical analysis indicated that the tested drug could restrain the growth of blood vessels in a dose-dependent manner. CONCLUSION: The method and the system are reliable for the analysis and can provide quantitative data from CAM model. It is also better than conventional method of blood vessel count from CAM images by microscope in analysis speed, precision and data reproducibility. Ju LH, Hu P, Ni S.Quantitative analysis of blood vessels in chick embryo chorioallantoic