胃肠舒片对大白鼠胃肠平滑肌细胞线粒体跨膜电位的影响_医学论文.docVIP

胃肠舒片对大白鼠胃肠平滑肌细胞线粒体跨膜电位的影响_医学论文 胃肠舒片对大白鼠胃肠平滑肌细胞线粒体跨膜电位的影响_医学论文 作者：宋晓冬 刘孟安 孙丰润 刘颖 张丽霞 牛新华 闫苗苗 王晓江 【摘要】 　　目的 研究胃肠舒片对大白鼠胃肠平滑肌细胞线粒体跨膜电位的影响。方法 胃肠平滑肌细胞离体培养，分为5组：空白对照组、西沙必利组（0.51 mg/ml）、胃肠舒Ⅰ组（25 mg/ml）、胃肠舒Ⅱ组（50 mg/ml）、胃肠舒Ⅲ组（75 mg/ml），采用Celltiter法和若丹明荧光法分别检测了胃肠舒片对大白鼠胃肠平滑肌细胞ATP和线粒体跨膜电位的的影响。结果 胃肠舒片作用24 h后与对照组和西沙必利组相比能显著增高平滑肌细胞ATP的含量（0.05），随着胃肠舒用药浓度的增高ATP的含量也增高；流式细胞仪结果表明胃肠舒明显加大了胃肠平滑肌细胞的线粒体跨膜电位，并且随着胃肠舒用药浓度的增高，跨膜电位也增高。结论 胃肠舒可能通过改变胃肠平滑肌细胞线粒体的跨膜电位促进了胃肠运动。 【关键词】 中药胃肠舒片　ATP　线粒体跨膜电位 　　【Abstract】 Objective To study the effect of Weichangshu on the mitochondrial potential of gastrointestinal smooth muscle cells.Methods Gastrointestinal smooth muscle cells were divided into 5 groups, as following.①Control group: cultured in normal medium.②Cisapride group: cultured in normal medium with 0.51 mg/ml Cisapride.③Weichangshu Ⅰ group: cultured in normal medium with 25 mg/ml Weichangshu.④Weichangshu Ⅱ group: cultured in normal medium with 50 mg/ml Weichangshu.⑤Weichangshu Ⅲ group: cultured in normal medium with 75 mg/ml Weichangshu. CelltiterGloTM was used to detect ATP. The relative changes in mitochondrial potential were tested with rhodamine fluorescence (R123) technique. Results After exposure to Weichangshu for 24 h, the change of ATP content was more significant (0.05) and the intensity of R123 fluorescence in gastrointestinal smooth muscle cells was significantly increased compared with those in the control group and cisapride group. With the concentration of Weichangshu increasing, the content of ATP and mitochondrial potential increased too.Conclusion Weichangshu might change the mitochondrial potential of gastrointestinal smooth muscle cells to improve gastrointestinal motility. 　　【Key words】 traditional Chinese medicine Weichangshu tablet, ATP, mitochondrial potential 　　胃肠运动障碍型疾病，是临床常见病，据国内统计约占胃肠专科门诊患者1/3以上，西方国家患病率高达20%～40%。现临床最常用的促胃肠动力药有胃复安、吗丁啉、西沙必利等化学药，化学药促进胃肠蠕动虽然有较好疗效，但其副作用如药理作用受限、催乳素增加、焦虑、激动、运动性不安、共济失调、腹泻、腹痛等不可忽视，且易于复发。而取之于天然的中药制剂由于具有治疗与调理双层作用，避免了化学药的毒副作用，人体不会对中药产生依耐性，服用安全